Evotec Biosystems Ag

Jan Bieschke

Manfred Eigen

Detlev Riesner

Andre Koltermann

Ulrich Kettling

Martin Pitschke

Jens Stephan

Petra Schwille

Oliver Schaefer

Klaus D&ouml;rre

Karin Post

Thorsten Winkler

Method for measuring the association of substructures of pathological protein depositions

The invention relates to a method for detecting reactions and conformational changes of analytes in a sample by coincidence analysis, as follows: the sample is marked with at least two different fluorescent dyes and then illuminated with at least one laser in order to stimulate the emission of fluorescence; the fluorescence signals are detected by at least two detection units and each signal is broken down into any simultaneous time segments with the desired time slot widths; the number of signals contained in at least one time segment and/or the time intervals between signals in the time segments are detected; a coincidence analysis of the data detected is carried out for at least one time segment of the first detection unit with at least one isochronous time segment of the second detection unit; statistics are produced showing the results of the coincidence analysis and/or the results are subjected to a threshold value analysis, and these statistics or a combination of more statistics are evaluated for the presence of characteristic features.

Method for detecting reactions by means of coincidence analysis

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Goettingen, Germany

Jan Bieschke