Roche Sequencing Solutions, Inc.

Ventana Medical Systems, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Jan Berka

Sedide Ozturk

Seoyoung Kim

Brian Christopher Godwin

Joseph Platzer

Carolina Dallett

Florian Rubelt

Yann A Astier

Ulrich Schlecht

Khai Luong

Ivona Voracova

Frantisek Foret

Richard Dannebaum

Vladimira Datinska

Jainee Lewis

Jigar Patel

Stacey Stanislaw

Nelson Alexander

Aoune Barhoumi

Rui Chen

Toumy Guettouche

Lisa L Gallegos

Maeve E O'Huallachain

Sri Paladugu

Dilduz Telman

Toummy Guettouche

Yann Astier

Nelson R Alexander

Maeve E O'HUALLACHAIN

The present disclosure generally relates to devices and methods for effecting epitachophoresis. Epitachophoresis may be used to effect sample analysis, such as by selective separation, detection, extraction, and/or pre-concentration of target analytes such as, for example, DNA, RNA, and/or other biological molecules. Said target analytes may be collected following epitachophoresis and used for desired downstream applications and further analysis.

Devices for sample analysis using epitachophoresis

Methods and compositions are described herein for primer extension target enrichment of immune receptor (BCR or TCR) sequences.

Immune profiling by primer extension target enrichment

Immuno-PETE

The present disclosure generally relates to devices for effecting epitachophoresis. Epitachophoresis may be used to effect sample analysis, such as by selective separation, detection, extraction, and/or pre-concentration of target analytes such as, for example, DNA, RNA, and/or other biological molecules. Said target analytes may be collected following epitachophoresis and used for desired downstream applications and further analysis.

The invention is a method of identifying a cognate antigen for a T-cell receptor using neoantigens from a patient's tumor cells combined with the patient's T-cells and using cell sorting, genome sequencing, expressing TCR genes, presenting tumor neoantigens on MHC complex and uniquely barcoding the T-cells where TCR recognition occurs to tag all components of the TCR recognition complex.

System for identification of antigens recognized by T cell receptors expressed on tumor infiltrating lymphocytes

Sample collection sump tray for multiwell plates

Methods, compositions, and reaction mixtures are provided for identifying a T cell receptor (TCR) and an epitope peptide that specifically binds the TCR. Methods, compositions, and reaction mixtures are also provided for identifying a plurality of T cell receptors and corresponding epitope peptides that specifically bind the T cell receptors. In some cases, the plurality of T cell receptors and corresponding epitope peptides can be identified in a highly parallel manner.

Identification of antigen epitopes and immune sequences recognizing the antigens

Growing community of inventors

Pleasanton, CA, United States of America

Jan Berka