454 Life Sciences Corporation

Roche Molecular Systems, Inc.

Curagen Corporation

Jan Berka

Jonathan Marc Rothberg

John Harris Leamon

Michael Weiner

Gary James Sarkis

Yi-Ju Chen

Kenton L Lohman

Vinod B Makhijani

John W Simpson

Maithreyan Srinivasan

Joel S Bader

Keith McDade

Scott B Dewell

Steve Lefkowitz

Christopher M Colangelo

Steven M Lefkowitz

Jonathan M Rothberg

Michael Egholm

Jan Fredrik Simons

Marie Carmen Ruiz-Martinez

Brian Christopher Godwin

Maithreyn Srinivasan

Zhoutao Chen

Stephen Kyle Hutchison

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

Bead emulsion nucleic acid amplification

The present invention provides for a method of preparing a target nucleic acid fragments to produce a smaller nucleic acid which comprises the two ends of the target nucleic acid. Specifically, the invention provides cloning and DNA manipulation strategies to isolate the two ends of a large target nucleic acid into a single small DNA construct for rapid cloning, sequencing, or amplification.

Paired end sequencing

Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (&#x3e;10,000) of different oligonucleotides.

Apparatus and method for sequencing a nucleic acid

Method of sequencing a nucleic acid

Disclosed herein are methods and apparatuses for sequencing a nucleic acid. In one aspect, the method includes annealing a population of circular nucleic acid molecules to a plurality of anchor primers linked to a solid support, and amplifying those members of the population of circular nucleic acid molecules which anneal to the target nucleic acid, and then sequencing the amplified molecules by detecting the presence of a sequence byproduct such as pyrophosphate.

This invention relates to methods and formulations for the separation of biological macromolecules according to their size using capillary electrophoresis with improved polyacrylamide matrixes under denaturing conditions.

Methods and formulations for the separation of biological macromolecules

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New Haven, CT, United States of America

Jan Berka