Ge Healthcare Bio-sciences Ab

Ge Healthcare Bioprocess R&d Ab

Amersham Pharmacia Biotech Ab

Pharmacia Biosensor Ab

Cytiva Bioprocess R&d Ab

Pharmacia Biotech Ab

Jan Bergstrom

Bo-Lennart Johansson

Gunnar Glad

Jean-Luc Maloisel

Nils Norrman

Tobias Soderman

Lennart Soderberg

Rolf Berglund

Bo Johnsson

Andreas Axen

James Van Alstine

Makonnen Belew

Henrik Ihre

Stefan Lofas

Anders Ljunglof

Stefan Lofaas

The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.

Chromatography medium

The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.

Separation matrix

The present invention relates to a method for running ion exchange chromatography on a media comprising shell beads having an inner porous core and an outer shell, wherein the inner core is provided with ligands whose charge changes with pH and the shell is provided with charged ion exchange ligands, the method comprising the following steps: a) adsorbing sample molecules on the shell ligands at a first pH; b) causing a discharge of the inner core ligands at a second pH by addition of a buffer substance that is able to increase its charge having the same sign/type as that of the core ligands, which at the same time causes release of ions from the inner core ligands and thereby an increase in ionic strength that displaces the sample molecules from the shell ligands i.e. causes an elution.

Method for ion-exchange chromatography and media used thereof

The present invention relates to a method and means to produce chromatography media having improved pressure-flow properties. More closely, the invention relates to bimodal particle size distribution and the use of layer functionalization as means to change pressure-flow properties of chromatography media. The invention relates to a method for production of chromatography media having improved pressure-flow properties, comprising mixing large beads/particles, comprising an inner core and an outer functionalized shell/lid, with smaller beads/particles, wherein the ratio of the particle size of large and small beads: [Dfor large particles/Dfor small particles]&#x3e;1.2, and wherein the volume ratio of large and small beads in the column: [Total volume of large beads/Total volume beads] is in the range 0.05-0.9.

Method for production of chromatography media

The present invention relates to a method of producing novel chromatography media and use thereof for purification of biomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially to advantageous for production of chromatofocusing media.

The present invention relates to a separation matrix comprised of a support to the surfaces of which polymer chains have been coupled, wherein each polymer chain presents recurring proton-donating groups and at least the surface of the support is substantially hydrophilic. In the most advantageous embodiment, the support is porous cross-linked agarose, the polymers are poly(acrylic acid) and the proton-donating groups are carboxyl groups. The matrix is useful e.g. to remove PEG from pegylated and/or native compounds in a liquid. Accordingly, the invention also encompasses a method, such as a chromatographic method, wherein the separation matrix according to the invention is used, for example as a pre-treatment of a reaction mixture that comprises unreacted PEG, pegylated proteins and native proteins.

Matrix for separation of polyethers and method of separation

The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.

Separation matrix having a ligand density gradient

Process for, in layers of a porous matrix, exhibiting the groups A, introducing a functionality using a reagent I, introducing the functionality by a reaction with the groups A. The characterizing feature is that the matrix is contacted with a functional deficiency of reagent I and that conditions and reagent I are chosen so that the reaction between reagent I and the groups A is more rapid than diffusion of reagent I in the matrix.

Process for introducing a functionality

Process for chromatographic separation of peptides and nucleic acid, and

A liquid chromatographic system comprises at least two modules, at least one fluid flow line, and at least two connector devices provided along the flow line and respectively connecting the modules to the flow line. One of the two modules comprises a separation module and the other of the two modules comprises an ancillary module having a non-separation function. Each of the two modules includes at least one end connector fit into the respective connector device, and each end connector is adapted to interchangeably fit into either connector device.

Liquid chromatographic system

A matrix coating suitable for use in a biosensor is provided. This matrix coating comprises a hydrogel bound to a surface and via which a desired ligand can be bound. This hydrogel is activated to contain charged groups for bringing about the concentration of biomolecules carrying an opposite charge to that of said charged groups, and reactive groups for covalently binding the biomolecules concentrated to the matrix coating.

Growing community of inventors

Balinge, Sweden

Jan Bergstrom