Ge Healthcare Bio-sciences Ab

Amersham Biosciences Ab

Pharmacia Biotech Ab

Intermag Gmbh

Jan Berglof

Ola Lind

Ingrid Drevin

Raf Lemmens

Joachim Stadler

Michael Katzke

Per Vretblad

Ulf Segerbo

Tomas Nyhammar

Ralf Helmut Nendza

The present invention relates to a method of sterilizing a chromatography column which is packed with a separation matrix, which method comprises passing a superheated aqueous liquid through the packing of the column to obtain at least one substantially sterile packed chromatography column. The method may also comprise a preceding heating. In the most advantageous embodiment, the superheated aqueous liquid is passed in a substantially uniform flow through the packing of the column, resulting in a sterility of the packed column.

Method of sterilization

The present invention relates to a process for separating nucleic acid molecules, preferably open circular and supercoiled plasmid DNA and RNA molecules from each other, comprising the steps of providing a solution comprising the molecules; adsorbing the molecules to adsorbing groups on a carrier; and optionally washing the column with a suitable solution. The present process is especially suitable for large-scale isolation of supercoiled ccc DNA to be used in gene therapy.

Isolation of DNA molecules

The present invention relates to a method for the removal of polyphenols and proteins simultaneously from a beverage by contacting the beverage with an ion exchanger that is capable of adsorbing both types of substances. The characteristic feature of the ion exchanger to be used is that it is a water-insoluble porous hydrophilic matrix to which ion exchanging groups are covalently bound.

Method for stabilizing a beverage containing haze causing substances

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Uppsala, Sweden

Jan Berglof