Oxford Nanopore Technologies Limited

Sharp Life Science (Eu) Limited

Price Industries Limited

James White

Andrew John Heron

James Anthony Clarke

Clive Gavin Brown

Lakmal Nishantha Jayasinghe

Daniel John Turner

Elizabeth Jayne Wallace

Rebecca Victoria Bowen

John Milton

Mark Bruce

Graham Hall

Daniel Ryan Garalde

Marion Louise Crawford

Gavin Harper

Ruth Moysey

David Jackson Stoddart

Richard George Hambley

Jonathan Bankes Pugh

Christopher Peter Youd

Daniel George Fordham

John Hagan Pryce Bayley

Stuart William Reid

Szabolcs Soeroes

Neil Roger Wood

Stephen Cheley

David Alves

Joseph Hargreaves Lloyd

Benjamin James Hadwen

Christopher James Wright

Sally Anderson

Hagan Bayley

Brian McKeown

Domenico Caprotti

Richard Alexander Gutierrez

Jason Robert Hyde

Luke McNeill

Matthew Holden

Ramiz Iqbal Nathani

Mike Holland

Mihaela Misca

Roger Charles Gill

James Edward Graham

Paul Richard Moody

Richard Muscat

Sylvio Tessier

Nicholas Antony Smith

Julian Rimmer

Jordan Hiebert

Mike Nicholson

Luke Marvin

Jessica Mary May Johnson

Sherwin Barroga

Arun Rabbani

Ashley Morgan

Jessica Mary May Knott

Craig Comberbach

Christian Borja

Ryan Sorrell

Antonino Puglisi

Mario Ortiz

Brian Mckeown

Jayne Wallace

David Antoni Alves

Andy Heron

Mark John Bruce

Stuart Reid

Rebecca Bowen

Julian Douglas Rimmer

Etienne Raimondeau

The invention relates to a method of characterising a target polynucleotide using a single-stranded binding protein (SSB). The SSB is either an SSB comprising a carboxy-terminal (C-terminal) region which does not have a net negative charge or a modified SSB comprising one or more modifications in its C-terminal region which decreases the net negative charge of the C-terminal region.

SSB method

Droplet interfaces are formed between droplets in an electro-wetting device comprising an array of actuation electrodes. Actuation signals are applied to selected actuation electrodes to place the droplets into an energised state in which the shape of the droplets is modified compared to a shape of the droplets in a lower energy state and to bring the two droplets into proximity. The actuation signals are then changed to lower the energy of the droplets into the lower energy state so that the droplets relax into the gap and the two droplets contact each other thereby forming a droplet interface. The use of sensing electrodes in the device permit electrical current measurements across the droplet interface. The sensing electrodes can be used for either (i) applying a reference signal during droplet actuation or (ii) recording electrical current measurements.

Droplet interfaces in electro-wetting devices

The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Dda helicase. The helicase controls the movement of the target polynucleotide through the pore. The invention also relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.

Modified helicases

The invention relates to a new method of characterising a target polynucleotide using a pore. The method involves controlling the formation of secondary structure by the target polynucleotide after the polynucleotide has moved through the pore.

Methods of characterising target polynucleotides

The invention relates to a new method of sequencing a double stranded target polynucleotide. The two strands of the double stranded target polynucleotide are linked by a bridging moiety. The two strands of the target polynucleotide are separated using a polynucleotide binding protein and the target polynucleotide is sequenced using a transmembrane pore.

Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores

The invention relates to a new method of determining the presence, absence or characteristics of an analyte. The analyte is coupled to a membrane. The invention also relates to nucleic acid sequencing.

Coupling method

The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNA polynucleotide has been modified to increase DNA helicase binding thereto.

Method for nanopore RNA characterization

A time-ordered series of measurements of a polymer made during translocation of the polymer through a Nanopore are analysed. The measurements are dependent on the identity of k-mers in the Nanopore, a k-mer bring k polymer units of the polymer, where k is a positive integer. The method involves deriving, from the series of measurements, a feature vector of time-ordered features representing characteristics of the measurements; and determining similarity between the derived feature vector and at least one other feature vector.

Analysis of measurements of a polymer

Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.

Method of characterizing a polynucleotide

The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing.

Enzyme-pore constructs

The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

Method for nucleic acid detection by guiding through a nanopore

The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins.

Method of improving the movement of a target polynucleotide with respect to a transmembrane pore

The invention relates to new methods of attaching one or more polynucleotide binding proteins to a target polynucleotide. The invention also related to new methods of characterising target polynucleotides.

Method for attaching one or more polynucleotide binding proteins to a target polynucleotide

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Oxford, United Kingdom

James White