Baxter International Inc.

James W Yang

T Michael Dennehey

Joseph C West, Jr

Arnold C Bilstad

David V Bacehowski

Herbert M Cullis

William C Brown

Martha C Pattillo

A bone marrow collection kit is provided comprising a collection container for receiving a bone marrow containing fluid having a body that defines an interior and an inlet opening and an outlet opening and including a prefilter that extends from the outlet opening into the interior of the collection container so that fluid must flow through the prefilter to exit the collection container through the outlet opening. A first inline filter member is coupled to the outlet opening of the collection container, the first inline filter member having a flexible plastic body that defines an interior, and an inlet opening and an outlet opening and the interior including a filter so constructed and arranged that fluid that enters the inlet opening must flow through the filter to exit the first inline filter member through the outlet opening. A container for receiving a resultant filtered bone marrow containing fluid having an interior in fluid communication with the outlet opening of the first inline filter member is also provided. Methods for processing the bone marrow and constructing the inline filter are also provided.

Bone marrow kit

Inline filter

A system for metering nutrient media to cell culture containers includes a media flow conduit which defines at one end means for substantially aseptic communication with the source of the media. In a preferred embodiment, sterile connectors may be used for essentially sterile conditions. A branch conduit extends from the flow conduit at a location spaced from the ends. The branch conduit communicates with means for receiving a predetermined-volume aliquot of media through the media flow conduit from said one end. The flow conduit also defines a portion downstream from the branch conduit, which communicates with at least one cell culture container, each container being a flexible bag. At least part of the flexible bag is made of a material capable of providing at least 2 times the oxygen diffusion and at least 2 times the carbon dioxide diffusion between the bag interior and exterior, compared with an identically-sized, sealed bag having polyethylene walls 0.013 inch thick.

Method for metering nutrient media to cell culture containers

System for metering nutrient media to cell culture containers and method

Growing community of inventors

Vernon Hills, IL, United States of America

James W Yang