Promega Corporation

James Unch

Poncho Meisenheimer

Keith V Wood

Brock Binkowski

Lance P Encell

Mary P Hall

Monika G Wood

Dieter Klaubert

Matthew B Robers

Thomas A Kirkland

Thomas Machleidt

Said A Goueli

Melanie Dart

Andrew S Dixon

Nidhi Nath

Marie Schwinn

Hicham Zegzouti

Dileep K Pulukkunat

Subhanjan Mondal

Wenhui Zhou

Michael P Valley

John William Shultz

James J Cali

Erika Hawkins

William Daily

Jianquan Liu

Michael Scurria

Hui Wang

Michael R Slater

Andrew L Niles

John W Schultz

Kevin Kupcho

Dieter H Klaubert

Brock F Binkowski

Erica Hawkins

Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Activation of bioluminescence by structural complementation

An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-typeluciferase.

Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

Activation of bioluminescene by structural complementation

Described are pro-coelenterazine analogues, methods for making the analogues, kits comprising the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays or fragment complementary luciferase. The disclosed pro-coelenterazine analogues provide increased serum stability for live cell assays, and are capable of tuning the brightness and assay windows as needed for the applications.

Serum stable pro-coelenterazine analogues

A method to detect the presence or amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.

Luminogenic and fluorogenic compounds and methods to detect molecules or conditions

A method to detect the presence of amount of at least one molecule in a sample which employs a derivative of luciferin or a derivative of a fluorophore is provided. Compounds and compositions for carrying out the methods of the invention are also provided.

The present invention provides, among other things, imidazo[1,2-&#x3b1;]pyrazine derivatives including The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules.

Substituted imidazo[1,2-a]pyrazines for use in bioluminogenic methods

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Arroyo Grande, CA, United States of America

James Unch