Powderject Vaccines, Inc.

Other

James T Fuller

Deborah L Fuller

Joel R Haynes

Georg Widera

Timothy Shipley

Mary Wu

A nucleic acid construct comprising a chimeric promoter sequence and a cloning site for insertion of a coding sequence in operable linkage with the chimeric promoter, wherein the chimeric promoter sequence comprises: (a) a hCMV immediate early promoter sequence; (b) exon 1 and at least a part of exon 2 of the hCMV major immediate early gene; and (c) a heterologous intron provided in place of the intron A region of the hCMV major immediate early gene.

Nucleic acid constructs

Reagents useful in nucleic acid immunization techniques are described. More particularly, adjuvanted genetic vaccine compositions are described, as are methods of using those compositions for inducing an enhanced immune response against a selected antigen.

Advanced genetic vaccines

Adjuvanted genetic vaccines

Recombinant nucleic acid molecules are described. The molecules have a first sequence encoding a Hepatitis B virus core antigen and a second sequence encoding at least one T cell epitope inserted within the first sequence. Vectors and compositions containing these molecules are also described. Methods of eliciting an immune response using these molecules are also described.

There is disclosed herein a spring battery retainer that is used to secure a small 'coin' or 'button' battery to a printed circuit (PC) card or the like. Use of the spring battery retainer provides for positive location of the battery, inexpensive electrical contacts for the battery terminals and positive spring compression that is necessary to insure hard contact with the cathode and the anode terminals of the battery. The battery is easily installed and removed by slightly lifting one end of the battery retainer and sliding the battery in or out. A bend in the spring battery retainer provides sufficient hold-down pressure so that the battery makes reliable contact with an electrical pad located on a PC board. The spring battery retainer is inserted into the PC card and then soldered to it in much the same way as a standard electrical component such as a resistor. The dimensions of the spring battery retainer can be varied so that coin or button batteries of various sizes can be securely fastened to a PC card.

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Madison, WI, United States of America

James T Fuller