Monsanto Technology LLC

James T Engleman

William P Donovan

John W Pitkin

Judith C Donovan

James Arthur Baum

Thomas M Malvar

James Kevin Roberts

Karina Krasomil-Osterfeld

The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space fromand related strains. The proteins are isolated from culture supernatants ofand related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle () and Southern Corn Rootworm (). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

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The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space fromand related strains. The proteins are isolated from culture supernatants ofand related strains and display insecticidal activity against lepidopteran insects including European corn borer (ECB), tobacco budworm (TBW) and diamondback moth (DBM). Insecticidal proteins encoded by nucleotide sequences that hybridize under stringent conditions to the isolated and characterized nucleotide sequences are disclosed. Methods are disclosed for making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

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Ephrata, PA, United States of America

James T Engleman