Repligen Corporation

W. R. Grace Co.-conn.

James Ronald Peyser

Alan Kit Ho Chan

James Neil Pryor

Feng Gu

James R Rusche

James M Bogdanor

Dennis McCreary

Thomas Pauly

Travis R Ward

Surya Kiran Chodavarapu

Dana Cristina Pentia

Evan Frankel

Dana Pentia

Methods and systems for removing gases and/or pressure formed during the sterilization, e.g., the gamma irradiation, of prepacked chromatography systems (column plus attached tube and valve set) are described. The methods include purging the gas and/or pressure through specially designed tube and valve sets without breaching the sterility of the prepacked sterile chromatography system. The systems include a sterile or aseptic pre-packed chromatography column including a column having an inlet and an outlet, a tubing and valve set attached to the inlet and the outlet, and a pump configured to pump sterile or aseptic liquid from the fluid source along the tubing and valve set, into the column tube inlet and out of the column tube outlet along a first flow path, thereby removing any entrapped gas and/or pressure from the chamber.

Methods and systems for removing pressure and air from chromatography columns

Chromatography media and devices containing chromatography media are disclosed. Methods of making chromatography devices and methods of using chromatography devices containing the chromatography media are also disclosed.

Affinity chromatography media and chromatography devices

If one sterilizes pre-packed, plastic chromatography columns with an appropriate level of gamma irradiation, the resulting sterile chromatography columns maintain sufficient packing media function and maintain column mechanical properties and pressure ratings.

Sterilizing chromatography columns

Disclosed are new recombinant nucleic acids encoding protein A polypeptides and methods of using these nucleic acids.

Nucleic acids encoding recombinant protein A

Growing community of inventors

Billerica, MA, United States of America

James Ronald Peyser