Leland Stanford Junior University

Genentech, Inc.

Greenlight Biosciences, Inc.

Other

University of Texas System

James Robert Swartz

Woon-Lam Susan Leung

Yuan Lu

Dong-Myung Kim

Nancy C McFarland

Alexei M Voloshin

Michael Christopher Jewett

John C Joly

Kara Calhoun

Judy Y Chang

Jessica Wuu

Phillip Richard Smith

George Georgiou

Jennifer R Cochran

Paul Henry Bessette

Ronald Levy

William Jeremy Blake

James M Williams

Bob Chen

Junhao Yang

Alfred Michael Spormann

Steven H Bass

Wei Min Chan

Shoshana Levy

Chia-Wei Wang

Ji Oiu

Rinchu Mathew

Christopher C Vanlang

Cem Albayrak

Amina Sheikh

Leonard H Sherman

Bertrand Howyen Lui

Marcus John Rohovie

Jennifer Lynn Lahti

Cheuk Lun Leung

Aaron R Goerke

Maya Nagasawa

Gregg Kanter

Bradley C Bundy

Marcus Emil Boyer

Jamin Koo

James Alan Stapleton

Kara Anne Calhoun

Susan Leung Woon-Lam

Genetically modified HBc polypeptides are provided.

Engineered hepatitis B core polypeptide

Methods and compositions are provided for the analysis of rare cells or other biological entities in a population, by contacting the population with a labeling nanoparticle comprising a VLP conjugated to a light emitting moiety and a specific binding moiety, then detecting the presence of bound nanoparticle by light emission.

Methods for enumeration of rare cells

Compositions and methods are provided for an Otolerant Fe&#x2014;Fe hydrogenase. The hydrogenases of the invention comprise specific amino acid substitutions relative to the native, or wild-type enzymes.

Oxygen tolerant hydrogenase by mutating electron supply pathway

The embodiments described herein pertain to cells, and methods for preparing cells, that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to a site where it is not in contact with the targeted enzyme in the intact cell. Upon cell lysis, the protease contacts the target enzyme, which is then inactivated by protease cleavage.

Recombinant cellular iysate system for producing a product of interest

Genetically manipulated cells, lysates of such cells, systems, and methods of use thereof are provided, where one or more enzymes in a pathway of interest are genetically modified to incorporate a peptide sequence that provides for relocation of the protein, e.g., to the periplasm, so as to sequester the enzyme, and where the enzyme controls flux in the pathway of interest.

Methods for control of flux in metabolic pathways through enzyme relocation

Stabilized hepatitis B core polypeptides

Vaccine compositions and methods of producing and using the same are provided, which compositions comprise a modified HA stem domain in a trimeric configuration.

Influenza vaccine constructs

The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.

Cell-free system for converting methane into fuel and chemical compounds

Intermolecular disulfide stabilized foldon polypeptides are provided.

Disulfide stabilized foldon polypeptides

Improved methods are provided in vitro synthesis of biological molecules, providing for improved yields, lowered costs, and enhanced utility. Improved yield and lowered cost is obtained by the use of a phosphate free energy source in the presence of exogenous phosphate, and optionally in the absence of exogenous nucleoside triphosphates.

Methods of in vitro protein synthesis

Bacterial flagellin protein is modified to improve adjuvant activity.

Ordered flagellin array as an immunostimulant

Methods are provided for a one step synthesis of polypeptide polymers or co-polymers. The polymers or co-polymers can be linear or branched. In the methods of the invention, the coding sequence for the polypeptide(s) to be polymerized is altered by introducing one or more codons for an nonnatural amino acid, which coding sequence is then utilized to produce the cognate polypeptide. The nonnatural amino acids are selected to be reactive with each other in a bioorthogonal reaction, and are combined in a conjugation reaction with the desired components of the polymer.

Synthesis of linear and branched polymers of polypeptides through direct conjugation

Compositions of a fusion protein comprising a spatially tethered ferredoxin-NADP-reductase (FNR) and an active [FeFe] hydrogenase, genetic sequences encoding such fusion proteins, and methods of use thereof are provided. The fusion proteins of the invention link an FNR polypeptide to an active [FeFe] hydrogenase through a polypeptide linker. The fusion protein facilitates improved electron transfer through a ferredoxin, and allows direct electron transfer from NADPH to the hydrogenase.

Hydrogenase fusion protein for improved hydrogen production

Methods for control of flux in metabolic pathways through protease manipulation

Growing community of inventors

Menlo Park, CA, United States of America

James Robert Swartz