Genzyme Corporation

Other

Purdue Research Foundation

Genzyne Corporation

James R Rasmussen

Gary D Barsomian

Barbara Y Wan

Jeffrey T Davis

Marianne Borowski

Michel Bergh

Marcelo Colon

Shirish Hirani

Jeffrey Davis

Joseph H Landry

Tracy L Johnnson

Robert L Van Etten

William R Gregor

William C Macom

John M Risley

A lifting apparatus for manipulating manhole castings and the like having at least one pinch clamp with an inner leg and an outer leg opposing one another wherein the inner leg and the outer leg are engageable with the manhole casting. A foot of the pinch clamp is connected to at least one of the inner leg and the outer leg wherein the foot is wider than the width of the inner leg and the outer leg and is engageable with the manhole casting. A flexible support is pivotally connected to the pinch clamp and is connectable to a lifting device wherein the foot and the legs of the pinch clamp are engageable with the manhole casting when the flexible support is pulled taut. Preferably, the inner and outer legs are separate structures pivotally connected thereto. Alternatively, the inner and outer leg may be integrally fabricated from a one-piece structure. The flexible supports are connected to a connector which is connectable to a lifting device.

Lifting apparatus for manipulating manhole castings and the like

Recombinant active glucocerebrosidase is produced by a eukaryotic cell. Also, a cell includes nucleic acid encoding enzymatically active glucocerebrosidase; also a eukaryotic organism contains such a cell. Also, a method for producing enzymatically active glucocerebrosidase includes steps of introducing glucocerebrosidase-encoding nucleic acid into a eukaryotic cell, causing the cell to express glucocerebrosidase, and purifying the glucocerebrosidase from the cell.

Enzymatically active recombinant glucocerebrosidase

This invention relates to a method of producing peracetyloxazolines from peracetyl saccharides. The method involves reacting the starting material, a peracetyl saccharide, with a reagent combination, to directly produce the peracetyl oxazoline. This method may be used for the activation of oligosaccharides, wherein an oligosaccharide containing a reducing GlcNAc terminus is activated by the formation of an oxazolide at the terminal GlcNAc, and then coupled with a bifunctional spacer to provide an oligosaccharide-spacer conjugate. The activated oligosaccharide-spacer conjugate is then coupled to a protein, such as granulocyte colony stimulating factor or .gamma.-interferon, providing a neoglycoprotein conjugate. The invention provides a method for forming neoglycoprotein conjugates which may improve biological and physiochemical properties of the protein. For example, serum lifetime or efficiency of drug delivery of the peptide to a target organ or cell may be improved.

Oligosaccharide oxazolines, oligosaccharide conjugates and methods of

A derivatized 1-amino-1-deoxyoligosaccharide prepared by reacting, at a pH of at least 6.5, a glycopeptide or glycoprotein containing one or more Asn-linked oligosaccharides with a .beta.-aspartylglycosylamine amidohydrolase, and contacting the products with an electrophilic reagent.

1-amino-1-deoxyoligosaccharides and derivatives thereof

The invention resides in oligosaccharide derivatives which are obtained by the enzynmatic cleavage of carbohydrates linked to proteins through asparagine groups. The oligosaccharide moieties of the instant invention are 1-amino-1-deoxyoligosaccharide compounds which are cleaved from a glycoprotein with an aminohydrolase enzyme, wherein the amino group of the oligosaccharide is subsequently substituted with a fluorescent reporter compound. The compounds of the instant invention are characterized by the glycosylamine bonds exhibiting less than 10% hydrolysis at 25.degree. C. in aqueous solution over a two days at pH values between 4-10.

Preparation of 1-amino-1-deoxyoligosaccharides and derivatives thereof

This invention relates to a method of producing peracetyloxazolines from peracetyl saccharides. The method involves reacting the starting material, a peracetyl saccharide, with a reagent combination, to directly produce the peracetyl oxazoline. This method may be used for the activation of oligosaccharides, wherein an oligosaccharide containing a reducing GlcNAc terminus is activated by the formation of an orazoline at the terminal GlcNAc, and then coupled with a bifunctional spacer to provide an oligosaccharide-spacer conjugate. The activated oligosaccharide-spacer conjugate is then coupled to a protein, such as granulocyte colony stimulating factor or .gamma.-interferon, providing a neoglycoprotein conjugate. The invention provides a method for forming neoglycoprotein conjugates which may improve biological and physiochemical properties of the protein. For example, serum lifetime or efficiency of drug delivery of the peptide to a target organ or cell may be improved.

An enzyme sample having Peptide-N.sup.4 -(N-acetyl-.beta.-N-glucosaminyl) asparagine Aminidase F (PNGase F) activity completely free from Endo-.beta.-N-acetylglucosaminidase F (Endo F) activity.

Endo F-free PNGase

Recombinant enzymatically active glucocerebrosidase is produced by a eukaryotic cell. Also, a cell includes nucleic acid encoding enzymatically active glucocerebrosidase; also a eukaryotic organism contains such a cell. Also, a method for producing enzymatically active glucocerebrosidase includes steps of introducing glucocerebrosidase-encoding nucleic acid into a eukaryotic cell, causing the cell to express glucocerebrosidase, and purifying the glucocerebrosidase from the cell.

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Cambridge, MA, United States of America

James R Rasmussen