Arkion Life Sciences, LLC

Bio-technical Resources, a Division of Arkion Life Sciences, LLC

Cargill Inc.

Bio-technical Resources, Inc.

Dcv, Inc.

Other

E.i. Dupont De Nemours and Company

Elevance Renewable Sciences, Inc.

Novus International, Inc.

Cultor Food Science, Inc.

Dcv Inc. Doing Business As Bio-technical Resourses

James R Millis

Julie Maurina-Brunker

Thomas W McMullin

Timothy Walter Abraham

David Paul Weiner

Douglas Conrad Cameron, Jr

Paula M Hicks

Lishan Zhao

Sara C McFarlan

John P Rosazza

Phillip Terry Olson

Gabriel G Saucy

John Anthony Hyatt

Michael John Tupy

Richard Paul Burlingame

Thomas F Blackburn

Richard P Burlingame

Alan D Grund

Mervyn L De Souza

James A Doncheck

Paul E Swanson

Michael H Reimer

Fernando Sanchez-Riera

Mary Ellen Rowland

Mark J Schendel

Michael Allen Eisenschink

Bruce Dexter King

Michael Alan Eisenschink

Alan Berry

Thomas W Mcmullin

A recombinant yeast that makes an isoprenoid compound is provided. The yeast comprises an endogenous mevalonate pathway comprising (i) an enzyme that converts acetyl-CoA to acetoacetyl-CoA, (ii) an enzyme that converts acetoacetyl-CoA to hydroxymethylglutaryl-CoA, (iii) an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid, (iv) an enzyme that converts mevalonic acid to phosphomevalonic acid, (v) an enzyme that converts phosphomevalonic acid to phosphomevalonate, and (vi) an enzyme that converts phosphomevalonate to isopentenyl pyrophosphate. The yeast further comprises heterologous nucleic acid sequences encoding an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid, an enzyme that converts mevalonic acid to phosphomevalonic acid, an enzyme that converts phosphomevalonic acid to phosphomevalonate, and an enzyme that converts phosphomevalonate to isopentenyl pyrophosphate. Carbon flow through the mevalonate pathway is increased in the recombinant yeast compared to a yeast that does not comprise the heterologous nucleic acid molecule encoding an enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid.

Production of isoprenoids

Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.

Polypeptides and biosynthetic pathways for the production of monatin and its precursors

The invention provides a biological method of producing isoprenoids.

A process for producing industrially important chemicals from renewable resources is disclosed. This 'biobased' process employs readily available, renewable resources comprising fatty acids rather than exploiting fossil sources, such as coal and petroleum. In one embodiment of the process 1-octene, along with methyl-9-decenoate and butadiene, is produced from linoleic acid via an enzymemediated isomerization reaction, followed by a metathesis reaction with ethylene. Linoleic acid can be isolated from vegetable oils, such as soybean oil.

Method for making industrial chemicals

The invention provides a biological method of producing farnesol or geranylgeraniol.

Production of farnesol and geranylgeraniol

The invention provides a biological method of producing geranylgeraniol.

Method of producing geranylgeraniol

Growing community of inventors

Kohler, WI, United States of America

James R Millis