Wyeth Research Ireland Limited

Genetics Institute, Inc.

Pfizer Ireland Pharmaceuticals

James R Mercer

Daniel T Lasko

Denis Drapeau

Wenge Wang

Yen-Tung Luan

Daniel R Lasko

Edward R LaVallie

Jeffrey S Deetz

Steven M Vicik

Neil L Schauer

Dwight Winters

Jenifer L Thomas

Catherine A Briasco

Edward R La Vallie

An improved system for large scale production of proteins and/or polypeptides in cell culture, particularly in media characterized by one or more of: i) a cumulative amino acid concentration greater than about 70 mM; ii) a molar cumulative glutamine to cumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine to cumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganic ion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined cumulative glutamine and cumulative asparagine concentration between about 16 and 36 mM, is provided. The use of such a system allows high levels of protein production and lessens accumulation of certain undesirable factors such as ammonium and/or lactate. Additionally, culture methods including a temperature shift, typically including a decrease in temperature when the culture has reached about 20-80% of it maximal cell density, are provided. Alternatively or additionally, the present invention provides methods such that, after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.

Production of anti-abeta

Production of TNFR-Ig

Production of polypeptides

Provided by the present invention are novel methods of protein recovery and purification methods and more specifically novel methods for the recovery and purification thioredoxin fusion proteins, especially of IL-11.

Protein recovery & purification methods

Growing community of inventors

Derry, NH, United States of America

James R Mercer