Baxter Diagnostics Inc.

Other

Dade Behring Inc.

James R Maynard

Pamela L Hawkins

Frederick Dombrose

Juan A Ruiz

According to the present invention highly specific-low affinity antibodies are generated which allow for the assay of F1.2 in bodily fluids that also contain prothrombin or other plasma proteins. Antibodies having the necessary properties for this assay are made using synthetic polypeptides which mimic the carboxy terminus of F1.2.

Immunoassay for F1.2 prothrombin fragment

The Prothrombin Time (PT) is used as a screening test for blood coagulation factor deficiencies and for monitoring oral anti-coagulant therapy using coumadin. Thromboplastin reagents activate the extrinsic pathway of coagulation and are the basis for the Prothrombin Time (PT) test. This invention describes the use of barium sulfate and chaotropic agents, and nonionic detergents, for the extraction of sensitive thromboplastin reagents from tissue. Extraction with sodium thiocyanate alone also greatly enhances thromboplastin sensitivity. This invention should be useful for all thromboplastins and will improve their sensitivity for all PT-based tests and specific assays.

Extraction methods for preparing thromboplastin reagents

Extraction method for preparing thromboplastin reagents

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Miami, FL, United States of America

James R Maynard