Roche Sequencing Solutions, Inc.

Genia Technologies, Inc.

Other

Roche Molecular Systems, Inc.

F. Hoffmann-la Roche Ag

James Pollard

Randall W Davis

Edward Shian Liu

Andrew Trans

Eric Takeshi Harada

Anne Aguirre

Cynthia Ann Cech

Hui Tian

John S Foster

Roger Chen

Robert A Yuan

Cynthia Cech

This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble.

Methods for forming lipid bilayers on biochips

A biochip for molecular detection and sensing is disclosed. The biochip includes a substrate. The biochip includes a plurality of discrete sites formed on the substrate having a density of greater than five hundred wells per square millimeter. Each discrete site includes sidewalls disposed on the substrate to form a well. Each discrete site includes an electrode disposed at the bottom of the well. In some embodiments, the wells are formed such that cross-talk between the wells is reduced. In some embodiments, the electrodes disposed at the bottom of the wells are organized into groups of electrodes, wherein each group of electrodes shares a common counter electrode. In some embodiments, the electrode disposed at the bottom of the well has a dedicated counter electrode. In some embodiments, surfaces of the sidewalls are silanized such that the surfaces facilitate the forming of a membrane in or adjacent to the well.

Process for biosensor well formation

Growing community of inventors

Mt. View, CA, United States of America

James Pollard