Waters Technologies Corporation

Other

Micromass UK Limited

New Objective, Inc.

Waters Technoligies Corporation

James P Murphy

Joseph D Michienzi

Michael O Fogwill

Geoff C Gerhardt

Dennis DellaRovere

Sylvain Gilles Cormier

Keith Fadgen

Moon Chul Jung

Michael John Tomany

Paul D Rainville

David Gordon

Wade P Leveille

Stevan Bajic

Jonathan L Belanger

Abhijit Tarafder

Russell Keene

David S Douce

Gary A Valaskovic

Robert Stephen Plumb

David P Prentice

Jennifer Harnisch Granger

Gary W Bertone

Jeffrey W Finch

Xiangjin Song

Gregory T Roman

John Chipperfield

Angela Doneanu

Ananya Dubey

Lee R Sawdey

John F Nethery

Thermally modulated variable restrictors used in chromatography systems enable independent control of system pressure and linear velocity of a compressible mobile phase passing through a chromatography column. A method for configuring a chromatography system with independent control of system pressure and mass flow rate of a compressible mobile phase includes determining a type of chromatography separation column to be used in the chromatography system, matching a thermally modulated variable restrictor to the type of chromatography separation column for use together during operation of the chromatography system, and bundling the chromatography column with its matching thermally modulated variable restrictor for distribution as a single package.

Matching thermally modulated variable restrictors to chromatography separation columns

The present disclosure relates devices and methods for analyzing intact antibodies, antibody subunits, antibody drug conjugate subunits, antibody drug conjugates, and intact proteins in a biological mixture.

Devices and methods for analyzing intact proteins, antibodies, antibody subunits, and antibody drug conjugates

Solvent containers and solvent container trays for chromatography systems are described for providing control over solvent supply and waste collection. Designated solvent containers and exclusively designated solvent containers provide solvents for use by chromatography systems. Control over solvent supply is achieved by requiring matched container shape and container receiving position shape within the tray, and additionally or alternatively, through solvent container coding readable by the solvent tray and chromatography system which provide information about the solvent container to the chromatography system.

Systems, methods, and devices providing solvent container identification and incorporation

Burner assemblies of flame-based detectors are configured to deliver decompressed mobile phase of supercritical fluid chromatography systems to the flame of a flame-based detector while providing for improved optimization of analyte response as well as enhanced flame stability during operation.

Flame ionization detection burner assemblies for use in compressible fluid-based chromatography systems

The present disclosure relates to an oxidizer, and related methods, for oxidizing polar modifiers in chromatographic mobile phases. The oxidizer enables the use of flame-based detection in chromatographic separations, such as carbon dioxide based chromatography, which employ polar modifiers, such as methanol. Upon exiting a chromatographic column, the mobile phase containing the polar modifier is flowed through an oxidizer that contains a catalyst to oxidize at least a portion of the polar modifier to a species that does not interfere with the function of the flame-based detector. The oxidizer allows for flame-based detection, such as flame ionization detection, in applications in which a polar modifier with a reduced form of carbon is used.

Catalytic oxidation of polar modifiers in chromatographic mobile phases

Burner assemblies are configured to deliver decompressed mobile phase of supercritical fluid chromatography systems to the flame of a flame-based detector while providing for improved optimization of analyte response as well as enhanced flame stability during operation.

A microfluidic device, for use in separation systems, includes a substrate having a fluidic channel. One or more heaters made of a thick film material are integrated with the substrate and in thermal communication with the fluidic channel of the substrate. The one or more heaters produce a thermal gradient within the fluidic channel in response to a current flowing through the one or more heaters. A plurality of electrically conductive taps can be in electrically conductive contact with the one or more heaters. The plurality of electrically conductive taps provides an electrically conductive path to the one or more heaters by which an electrical supply can produce the current flowing through the one or more heaters. Alternatively, the thick film material can be ferromagnetic, and the electrical supply can use induction to cause the current to flow through the one or more heaters.

Apparatus and methods for creating a static and traversing thermal gradient on a microfluidic device

An apparatus for use in a chromatography system includes a microfluidic substrate having a fluidic channel configured as an analytical chromatographic column and a fluidic port on one side of the microfluidic substrate. The fluidic port opens at a head end of the analytical chromatographic column. A dried blood spot (DBS) collection device holds one or more dried biological samples. The DBS collection device is directly coupled to the microfluidic substrate whereby one of the biological samples is placed into fluidic communication with the fluidic channel of the microfluidic substrate and an extraction of that biological sample flows toward the head end of the analytical chromatographic column. A diluent source fluidically coupled to the fluidic port supplies a solvent to the head end of the analytical column to dilute the extracted biological sample before the biological sample flows into the analytical chromatographic column.

Analysis of dried blood spot samples in a microfluidic system with dilution of extracted samples

A chromatography system includes a separation column that separates a sample carried by a compressible mobile phase flow into analytes and a splitter in fluidic communication with the separation column to receive and divide the compressible mobile phase flow into first and second mobile phase streams in accordance with a split ratio. A thermally modulated variable restrictor is coupled between the splitter and a detector. The restrictor receives the first mobile phase stream from the splitter and has a temperature element in thermal communication with the first mobile phase stream to exchange heat therewith. A controller, in communication with the restrictor, dynamically adjusts a temperature setting of the temperature element of the restrictor to adjust the heat exchange between the thermally modulated variable restrictor and the first mobile phase stream in order to keep the split ratio constant throughout a chromatographic run.

Thermally modulated variable restrictor for normalization of dynamic split ratios

An apparatus for chemical separations includes a first substantially rigid microfluidic substrate defining a first fluidic port; a second substantially rigid microfluidic substrate defining a second fluidic port; and a coupler disposed between the first and second substrates, the coupler defining a fluidic path in fluidic alignment with the ports of the first and second substrates. The coupler includes a material that is deformable relative to a material of the first substrate and a material of the second substrate. The substrates are clamped together to compress the coupler between the substrates and form a fluid-tight seal.

Chromatography apparatus and methods using multiple microfluidic substrates

The invention provides interfaces between analytical instruments, e.g., between chromatography systems and mass spectrometers. In an exemplary embodiment, an ion source is provided for connecting a carbon dioxide-based chromatograph device to a mass spectrometer. The ion source includes a first conduit for receiving eluent from the chromatography device, a heater for heating at least a portion of said first conduit, a second conduit in fluid communication with the first conduit, an inlet for receiving eluent from said second conduit and introducing the eluent into an ion source region to form a plume of gas and/or liquid in the ion source region, and an ionization promoting inlet for injecting an ionization promoting fluid into the ion source region to interact with the plume to promote ionization of at least some of the plume.

Systems, devices, and methods for connecting a chromatography system to a mass spectrometer

The present disclosure relates to a microfluidic flame ionization detector for use in small scale separations, such as, for example, microfluidic gas chromatography and microfluidic carbon dioxide based fluid chromatography. In some arrangements, the microfluidic counter-current flame ionization detector employs a non-parallel arrangement for the introduction of combustion gases into the combustion chamber. In other arrangements, the detector housing is configured to incorporate at least one of the detector electrodes within the housing using electrically isolating fittings.

Microfluidic flame ionization detector

An apparatus for use in a chromatography system includes a first microfluidic substrate having a first fluidic channel. One end of the first fluidic channel terminates at a first fluidic port on a first side of the first microfluidic substrate and an opposite end of the first fluidic channel terminates at a second fluidic port on a second side of the first microfluidic substrate. A second microfluidic substrate has a second fluidic channel. One end of the second fluidic channel terminates at a first fluidic port on a first side of the second microfluidic substrate. The first side of the second microfluidic substrate abuts the second side of the first microfluidic substrate such that the fluidic port of the second microfluidic substrate aligns with one of the fluidic ports of the first microfluidic substrate and the alignment produces a fluidic path comprised of the first and second fluidic channels.

Microfluidic device with dried blood spots (Dbs) card interface

The present disclosure relates to flame based detection methods for compressed mobile phase chromatography. In particular, the present disclosure relates to the operation of a flame ionization detector for carbon dioxide based chromatography, such as supercritical fluid chromatography. The present disclosure includes a method of matching a chromatographic column with a flame ionization detector inner burner including providing a chromatographic column with an internal diameter, determining an optimal mobile phase flow rate for the chromatographic column, calculating an optimal inner diameter of the inner burner that combined with the internal diameter and flow rate of the column produces optimal detector performance, and providing a flame ionization detector inner burner having an inner diameter substantially equal to the calculated optimal inner diameter.

Growing community of inventors

Franklin, MA, United States of America

James P Murphy