Miles Inc.

Bayer Corporation

Miles Laboratories, Inc.

Bayer Healthcare LLC

Molecular Diagnostics, Inc.

Other

Siemens Medical Solutions Diagnostics

Charlton, Steven C.

Albarella, James P.

Reinsch, James W.

Warchal, Mary E.

James P Albarella

Michael J Pugia

Robert P Hatch

David L Garling

J Oakey Noell

Steven C Charlton

Nan-Horng Lin

Robert J Carrico

Karen L Marfurt

Nanibhushan Dattagupta

Steven W Felman

Thomas M Li

Gary M Johnson

Paul O Vogelhut

Mary E Warchal

James W Reinsch

John J Landi

Robert T Buckler

Ronald G Sommer

Meitak Teresa Yip

Lloyd S Schulman

Frederick E Ward

Wallace G Strycker

Michael L Bloczynski

Angela A Michaels

Sally E Cahill

Karl-Heinz Hildenbrand

David W Michaels

Jennifer Farr

Lloyd A Schick

Frank Thomas Hartl

Nazeer H Shareef

Spencer H Lin

Linda M Anderson-Mauser

Leslie H Anderson

James E Smous

Deceased, Frederick Ward

Mietak T Yip

Todd K Cast

Frederick E Ward, Deceased

Thiazolyl tetrazolium salts with increased solubility in aqueous solutions have alkylammonium alkoxy substituents, preferably trimethyl ammonium propoxy groups substituents, on phenyl rings attached to the tetrazolium ring.

Process of using a tetrazolium salt

Thiazolyl tetrazolium salts with increased solubility in aqueous solutions have alkylammonium alkoxy substituents, preferably trimethyl ammonium propoxy groups substituents, on phenyl rings attached to the tetrazolium ring. Thiazolyl tetrazolium salts with increased solubility in aqueous solutions have alkyl ammonium alkoxy substituents, preferably trimethyl ammonium propoxy groups substituents, on phenyl rings attached to the tetrazolium ring, e.g. the compounds of the general formula shown below. Formula (A-). 

Water-soluble tetrazolium salts

A device for evaluating at least one analyte in a test sample. The device comprises a cassette having a hollow test chamber, a test strip disposed within the test chamber for receiving the test sample, and a temperature control member. The cassette has at least one aperture extending from an exterior of the cassette to the hollow test chamber. The test strip includes a reagent adapted to react with the at least one analyte to produce a reaction indicative of the presence of the analyte. The temperature control member is adapted to extend through the at least one aperture in the cassette and into the test chamber for controlling the temperature of the test chamber.

Assay test system for regulating temperature

A method of detecting creatinine in body fluids using an indicator which produces a fluorescent response when oxidized in the presence of a copper/creatinine complex. A preferred indicator is 4-(1-methylhydrazino)-7-nitro benzooxadiazole (MNBDH).

Fluorescent creatinine assay

One embodiment of the present invention improves the visual use of urine test strips and reagent pads by providing a single color from a reference color spectrum directly on the colorimetric strip and adjacent to the reagent area to allow easy comparison. After application of a fluid to be tested to a reagent area on the colorimetric strip, a technician is able to easily compare the color in the reagent area to the reference color area(s) to determine the presence or absence of, for example, glucose, in the fluid. Placing at least one reference color immediately next to the reagent area markedly improves technician's accuracy in comparing and analyzing the reagent area against the reference color(s). The present invention allows a technician to easily and quickly compare a reagent area color to more than one reference area to determine the actual numerical value of, for example, the pH in the tested fluid.

Method and apparatus for producing visual results using colorimetric strips

Disclosed is a diagnostic device for the colorimetric detection of an analyte in a test fluid. The device is a dry reagent layer which is overcoated with a transparent, fluid permeable membrane. The membrane is made up of a combination of a water dispersible and a water soluble polymer. The membrane may contain a surfactant and a thickener.

Transparent flow through membrane for dry reagent analytical devices

Disclosed is a method for cleaning optical surfaces, such as those in a urine analyzer, which surfaces come into repeated contact with urine test samples. The method involves contacting the optical surfaces with an aqueous solution of a quaternary ammonium or phosphonium salt, a non-ionic surfactant and a divalent ion.

Cleaning method for optical surfaces of urine analyzers

The determination of human serum albumin (HSA) in urine is carried out by contacting the urine with a reagent system containing a buffer and a protein error indicator. Other proteins normally found in urine can compete with the HSA for interaction with the protein error indicator thereby affecting the sensitivity of the test for HSA. The present invention involves the addition of certain polymeric competitive inhibitors to the reagent system which inhibit the interaction of the urinary proteins other than HSA with the protein error indicator to a greater extent than the interaction with HSA is inhibited thereby increasing the reagent's sensitivity for HSA. Suitable polymeric inhibitors include poly(vinyl alkyl esters), poly(alkyl acrylates), poly(vinyl alkyl carbonates) and poly(vinyl alkyl ketones).

Method for the detection for protein

Disclosed are novel folic acid-alkaline phosphatase conjugates useful in a competitive immunoassay for folic acid in a biological fluid such as serum. A preferred conjugate involves a folate hapten bound to (N-Succinimidyl�4-iodoacetyl!aminobenzoate) through a 1,12-diamino-4,9-dioxadodecanyl spacer arm.

Folate-alp conjugate

The detection of protein is accomplished using novel composition and method involving phenolsulfonephthalein protein error indicator, buffer and an aliphatic ether-polycarbonate present in an amount equal to or less than ten percent by weight.

Assay for the determination of protein in a biological sample

Test compositions, indicators, and test device are provided which are capable of generating different hues at different analyte concentrations. The compositions are capable of generating a yellow hue in situ. Visual tests for clinically important analytes, such as glucose, are determined by use of two independent catalytic systems which are reactive with reduced nicotinamide adenin dinucleotide to produce a range of hues; the particular hue produced depending on the concentration of the analyte. The invention provides a test device for the determination of analyte, e.g. glucose, in body fluid which exhibits a RAINBOW of hues, the particular final hue produced depending on the analyte concentration.

Dithiol-(2-nitrobenzoate) indicators

Disclosed is a novel diagnostic reagent system for the detection of hydroperoxides or substances which react with peroxidatively active substances resulting in the liberation of hydroperoxides which reagent system comprises a chromogenic oxidation indicator and a peroxidase or a peroxidatively active substance, together with an inhibitor of color generation selected from the group of 1,4-disubstituted semicarbazides, thiosemicarbazides and 1,5-disubstituted carbazides as inhibitors of color generation. By inhibiting the generation of color formed by oxidation of the chromogenic indicator, the indicator's usefulness in discriminating between varying concentrations of analyte is enhanced.

Use of inhibitors of color generation in chromogenic assays

Electrically conducting homo- and/or copolymers and/or tripolymers can be produced from novel monomers, such as a 3-substituted 2,5-di(2-thienyl)pyrrole. The polymers exhibit unexpectedly high stability and conductivities, and can be functionalized, such as with an enzyme, like glucose oxidase, or an ion-specific binding site, like a crown ether, or an antigen, without adversely affecting the conductivity of the polymer. The functionalized, conducting polymer can be used in a diagnostic device to determine the presence and concentration of a specific analyte in a liquid medium. For example, the presence and concentration of glucose is determined by measuring the conductivity change in the polymer caused by the vibrational excitation induced in the enzyme, glucose oxidase, from its reaction with the glucose and/or by measuring a secondary effect of the enzyme/substrate reaction, such as the change in the conductivity of the conducting polymer caused by the generation of hydrogen peroxide during the glucose-glucose oxidase reaction.

Substituted bithiophenes and dithienylpyrroles

The present invention relates to a chromogenic benzoisothiazolone polymer derivative for measuring the presence or appearance of thiols in an aqueous system.

Chromogenic thiol indicating benzoisothiazolone compounds

A process for measuring the presence or appearance of thiols in aqueous system, the process involving contacting the aqueous system with a chromogenic thiol indicating benzoisothiazolone derivative and then observing chromophoric changes due to thiol mediated reduction of the benzoisothiazolone derivative wherein the changes are proportional to the amount or rate of appearance of the thiols in the aqueous system.

Process for determining thiols using chromogenic benzoisothiazolone

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Granger, IN, United States of America

James P Albarella