University of Iowa Research Foundation

Integrated Dna Technologies, Inc.

James O McNamara

Mark Aaron Behlke

Lingyan Huang

Frank J Hernandez

Alexander R Horswill

Katie R Flenker

Richard Owczarzy

Karen Clark

Hyeon Kim

Paloma H Giangrande

Elliot Burghardt

William Thiel

Anton P McCaffrey

Katie R Stockdale

Kristina W Thiel

Alexander R Howswill

Elliott Burghardt

William M Rockey

In certain embodiments, the present invention provides a detection composition comprising a picodroplet comprising (a) an aqueous solution, and (b) a substrate probe comprising (i) an oligonucleotide of 2 to 75 nucleotides in length, (ii) a fluorophore operably linked to the oligonucleotide, and (iii) a quencher operably linked to the oligonucleotide. As used herein, the term 'picodroplet' comprises a liquid droplet that has a volume of 0.014 to 2.6 picoliters. In certain embodiments, the present invention provides a method of detecting at least one individual nuclease molecule present in a sample, comprising contacting an aqueous sample suspected of containing at least one nuclease with at least one detection composition comprising a picodroplet comprising (a) an aqueous solution, and (b) a substrate probe comprising (i) an oligonucleotide of 2 to 75 nucleotides in length, (ii) a fluorophore operably linked to the oligonucleotide, and (iii) a quencher operably linked to the oligonucleotide to form an aqueous reaction mixture; emulsifying the aqueous mixture in oil to form picoliter-scale droplets in an emulsion, (c) incubating the picoliter-scale droplets in the emulsion in order for the nuclease, if present, to digest the substrate probes linked to the microbeads; recovering the microbeads; and detecting fluorescence emitting from the microbeads.

Digital nuclease detection compositions and methods

The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., endonuclease) substrates, and probes and compositions useful in detection assays.

Oligonucleotide-based probes and methods for detection of microbes

The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., ribonuclease) substrates, and probes and compositions useful in detection assays.

Oligonucleotide-based probes for detection of bacterial nucleases

The present invention relates to a rapid detection of microbial-associated nuclease activity with chemically modified nuclease (e.g., ribonuclease) substrates, and probes and compositions useful in detection assays. Accordingly, in certain embodiments, the present invention provides a probe for detecting a microbial endonuclease comprising a substrate oligonucleotide of 2-30 nucleotides in length, a fluorescence-reporter group operably linked to the oligonucleotide, and a fluorescence-quencher group operably linked to the oligonucleotide. The fluorescence-reporter group and the fluorescence-quencher group are separated by at least one RNAse-cleavable residue, e.g., RNA base.

The present invention relates to optimized HER2 aptamers and methods of using these aptamers.

HER2 nucleic acid aptamers

The present invention relates to optimized aptamers and methods of using these aptamers.

Nucleic acid aptamers

Growing community of inventors

Iowa City, IA, United States of America

James O McNamara