Single Cell Technology, Inc.

X-ray Scanner Corporation

The Perkin-elmer Corporation

Bowlby Labs, Inc.

James O Bowlby, Jr

Chun-Nan Chen

Richard Aleck Jorgensen

Gregory E Johnston

Mark Jay Shlomchik

Robert E Malm

David R Nygren

Daniel B Allison

This invention generally relates to methods, devices and kits for screening a plurality of single secreting cells for functional activity of the secreted molecules by measuring the amount of reporter gene mRNA produced in one or more reporter cells in response to the secreted molecules.

Method of screening a plurality of single secreting cells for functional activity

The invention is a methodology which makes it possible to select from a very large number of cells, a single cell or cells of interest and obtain specific information from those cells in a rapid and efficient manner. As an example of the methodology, a large number of antibody producing cells such as plasma cells are separated so that these individual antibody producing plasma cells are placed in individual wells. The cells are allowed to produce antibodies and the antibodies in the wells are then contacted with a protein bound to a solid surface such as a well top. The protein universally and specifically binds antibodies in the wells. The surface or well tray top includes addresses configured such that each address is specifically related to one of the individual wells containing a cell producing antibodies.

Method of obtaining antibodies of interest and nucleotides encoding same

This invention generally relates to a methods, devices and kits for screening single cells for the production of one or more biologically active agents of interest, such as a protein, nucleic acid, or a protein and the nucleic acid encoding same.

Method of screening single cells for the production of biologically active agents

A multi-well system is described which is comprised of a tray comprising a plurality of wells which may include thousands, tens of thousands or even hundreds of thousands of wells or more. Each of the wells has a unique address. The system includes a tray top comprised of a plurality of areas wherein each of the areas corresponds uniquely to each of the wells and includes a unique address. The system may include a second tray top also comprised of a plurality of areas with unique addresses which uniquely correspond to each of the wells. The areas on the tops have compounds bound to those areas which compounds bind to components present in the wells and are used to obtain specific material in each well for analysis.

Multi-well system

An improved signal processing method in a signal processor for performing the deconvolution of a signal resulting from an analytical separation process is disclosed. In a first aspect, a signal representing a plurality of partially separated sample zones is measured, a point-spread-function of the signal is determined, and the Fourier transform of the signal and the point-spread-function is taken. Next, a noise component n of the signal is determined and the value of a result signal A(f) is calculated using the following filter ##EQU1## where D(f) is the Fourier transform of the signal, P(f) is the Fourier transform of the point-spread-function, and P*(f) is the complex conjugate of P(i). Finally, the inverse Fourier transform of the result A(f) is taken and reported as A(t). Preferably, the point-spread function is a Gaussian function having a standard deviation .sigma., where .sigma. is determined using either an .alpha..beta. tracker or the function .sigma.=(a+bt.sup.2).sup.1/2, where a and b are constants. In a second aspect of the invention, a plurality of possible point-spread-functions of the signal are determined and the above-described method is applied using each PSF. The value of the largest point-spread-function which provides a nonnegative result is determined, and the associated value of A(t) is reported. In a third aspect of the invention, a program storage device readable by a machine, tangibly embodying a program of instructions executable by a machine to perform the method steps of the first or the second aspects of the invention is provided.

Deconvolution method for the analysis of data resulting from analytical

A charge collection control circuit generates a time-varying voltage that is used to control the charge collection process in an MOS photodetector for the purpose of obtaining greater dynamic range. Control is exercised either by means of an integration control electrode that controls the flow of charge from a charge-collecting region to a sink or by means of a transfer electrode that controls the flow of charge from the charge-collecting region to a charge-transport region through which the collected charge flows to an output circuit.

Methods and apparatus for obtaining nonlinear responses from

The charge collection control circuit generates a time-varying voltage that is used to control the charge collection process in an MOS photodetector for the purpose of obtaining greater dynamic range. Control is exercised either by means of an integration control electrode that controls the flow of charge from a charge-collecting region to a sink or by means of a transfer electrode that controls the flow of charge from the charge-collecting region to a charge-transport region through which the collected charge flows to an output circuit.

Charge collection control circuit for use with charge-coupled

An apparatus for improved paper marking is provided which increases the percentage moisture content of the paper, thereby decreasing its electrical resistivity. The paper is then dried at selected pixels which results in high electrical resistivity of those pixels. Subsequently, the paper is charged with a high negative voltage. The areas with high moisture content rapidly discharge the applied charge, whereas the areas with low moisture content retain the charge. Finally, the areas with retained charge attract toner and are fused to the paper.

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San Jose, CA, United States of America

James O Bowlby, Jr