Newlink Genetics Corporation

Human Gene Therapy Research Institute

James N Higginbotham

Charles J Link, Jr

William J Ramsey

Mario Mautino

Nicholas N Vahanian

Tatiana Seregina

Won-Bin Young

Cherisa Meyer

W Jay Ramsey

Bradley J Powers

Sachet A Shukla

Won Bin Young

Teresa Di Colandrea

The invention includes a viral vector method and composition comprising transcomplementary replication incompetent viral vectors, preferably adenoviral vectors, which are cotransformed to a recipient cell. The two vectors complement each other and thus allow viral replication, in a synergistic combination which enhances both gene delivery and gene expression of genetic sequences contained within the vector.

Methods and compositions for efficient gene transfer using transcomplementary vectors

A method is described for the rapid identification and isolation of cells based on the presence or absence of an ectopically-expressed N-acetyllactosaminide 3-&#x3b1; Galactosyltransferase (&#x3b1;GT) enzyme for the production of &#x3b1;Galactosyl-(1,3)Galactosyl (&#x3b1;Gal) epitopes on the surface of &#x3b1;Gal-negative cells. These cells which are genetically modified to express the &#x3b1;GT enzyme and &#x3b1;Gal epitopes on glycosylated lipids and proteins of the cell surface are then labeled via an antibody composition which recognizes and binds the &#x3b1;Gal epitopes on the cell surface. Cells labeled with the anti-&#x3b1;Gal antibody can be isolated by sorting via fluorescence activated cell sorting (FACS), or by magnetic panning techniques. This method is suitable for the rapid positive or negative selection of &#x3b1;Gal-positive cells from within a population of &#x3b1;Gal-negative cells without the need to expose cells to antibiotics for any period of time. In addition, the specification provides a method for the production and purification of anti-&#x3b1;Gal antibodies from chicken egg yolk.

Selectable gene marker system based on expression of N-acetyllactosaminide 3-&#x3b1; galactosyltransferase

The present invention relates generally to methods and compositions for the identification of differential protein expression patterns and concomitantly the active genetic regions that are directly or indirectly involved in different tissue types, disease states, or other cellular differences desirable for diagnosis or for targets for drug therapy.

Methods and compositions for elucidating relative protein expression levels in cells

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Ames, IA, United States of America

James N Higginbotham