Verily Life Sciences LLC

James Michael Higbie

Supriyo Sinha

Cheng-Hsun Wu

Seung Ah Lee

Charles Santori

John D Perreault

Andrew Homyk

Victor Marcel Acosta

Chinmay Belthangady

Brian M Rabkin

Eden Rephaeli

Vikram Singh Bajaj

Kimberly Kam

Stephen Morton

Vasiliki Demas

Joshua Simon Klein

Huang-Wei Chang

Mark Audeh

Anthony M Giannetti

The present disclosure relates to systems and methods for cellular imaging and identification through the use of a light sheet flow cytometer. In one implementation, a light sheet flow cytometer may include a light source configured to emit light having one or more wavelengths, at least one optical element configured to form a light sheet from the emitted light, a microfluidic channel configured to hold a sample, and an imaging device. The imaging device may be adapted to forming 3-D images of the sample such that identification tags attached to the sample are visible.

Light sheet imaging flow cytometer

Systems and methods are described that enable sensing of magnetic fields within skin tissue. Specifically, a system includes one or more microneedles that include a high magnetic permeability material. The system also includes a magnetic sensor communicatively coupled to the microneedle and configured to detect a magnetic field proximate to the microneedle. The system also includes a controller configured to receive information indicative of a magnetic field proximate to a portion of the microneedle. The controller is further configured to determine a presence of at least one magnetic nanoparticle proximate to the portion of the microneedle based on the received information. Alternatively, other embodiments include a microneedle that includes a nanodiamond material configured to detect a local magnetic field. Such embodiments also include a light source configured to cause the nanodiamond material to emit characteristic emission light that may indicate at least a magnitude of the magnetic field.

In-vivo magnetic detection of magnetic nanoparticles using microneedles

Systems and methods for generating virtually stained images of unstained samples are provided. According to an aspect of the invention, a method includes accessing an image training dataset including a plurality of image pairs. Each image pair includes a first image of an unstained first tissue sample, and a second image acquired when the first tissue sample is stained. The method also includes accessing a set of parameters for an artificial neural network, wherein the set of parameters includes weights associated with artificial neurons within the artificial neural network; training the artificial neural network by using the image training dataset and the set of parameters to adjust the weights; accessing a third image of a second tissue sample that is unstained; using the trained artificial neural network to generate a virtually stained image of the second tissue sample from the third image; and outputting the virtually stained image.

Generating virtually stained images of unstained samples

Systems and methods for hyperspectral imaging are described. In one implementation, a hyperspectral imaging system includes a sample holder configured to hold a sample, an illumination system, and a detection system. The illumination system includes a light source configured to emit excitation light having one or more wavelengths and a diffractive element. The illumination system is configured to structure the excitation light into a predetermined two-dimensional pattern at a conjugate plane of a focal plane in the sample, spectrally disperse the structured excitation light in a first lateral direction, and illuminate the sample in an excitation pattern with the one or more wavelengths dispersed in the first lateral direction.

Systems and methods for 4-D hyperspectral imaging

Body-mountable devices are provided to detect the presence or status of an analyte in subsurface vasculature of a body by detecting fluorescent reporters that are bound to instances of the analyte in the subsurface vasculature. Such devices further operate to exert an attractive magnetic force on magnetic nanoparticle-containing probes that are configured to bind to the analyte, thus concentrating the analyte proximate the devices by magnetically exerting attractive forces on such probes that are bound to instances of the analyte. The analyte can be an extracellular vesicle that is characteristic of a cancer, and a body-mountable device as described herein could be used to detect such extracellular vesicles in a portion of subsurface vasculature such that a presence or status of a tumor could be determined based on an amount, presence, or other detected property of the extracellular vesicle.

Magnetic probes for in vivo capture and detection of extracellular vesicles

Systems and methods for hyperspectral imaging are described. In one implementation, a hyperspectral imaging system includes a sample holder configured to hold a sample, an illumination system, and a detection system. The illumination system includes a light source configured to emit excitation light having one or more wavelengths, and a first set of optical elements that include a first spatial light modulator (SLM), at least one lens, and at least one dispersive element. The illumination system is configured to structure the excitation light into a predetermined two-dimensional pattern at a conjugate plane of a focal plane in the sample, spectrally disperse the structured excitation light in a first lateral direction, and illuminate the sample in an excitation pattern with the one or more wavelengths dispersed in the first lateral direction.

Systems and methods are described that relate to a nanophotonic optical system. The nanophotonic optical system may be configured to transmit light in a range of wavelengths. The nanophotonic optical system includes at least one nanophotonic element, which includes a two-dimensional arrangement of sub-wavelength regions of a first material interspersed within a second material, the first and second materials having different indices of refraction. The at least one nanophotonic element includes a surface having a curvature and an optical phase transfer function dependent on the curvature of the surface. The nanophotonic optical system includes an actuator configured to modify the curvature of the surface and a controller. The controller is configured to determine a threshold optical phase transfer function and cause the actuator to modify the curvature of the surface to provide the threshold optical phase transfer function.

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Palo Alto, CA, United States of America

James Michael Higbie