Leland Stanford Junior University

James Marshel

Karl A Deisseroth

Methods for selectively stimulating a plurality of light-responsive neurons in a sample are provided. Methods according to certain embodiments include irradiating a sample comprising a plurality of light-responsive neurons with a plurality of holographic images that are each configured to stimulate one or more light-responsive neurons in the sample, wherein the holographic images are created by light projection system that includes a plurality of light sources; a plurality of optical adjustment components; a plurality of spatial light modulators; a controller; a processor; and a computer-readable medium comprising instructions that, when executed by the processor, cause the controller to operate the light sources, optical adjustment components and spatial light modulators to generate and display a plurality of holographic images; direct each of the holographic images to a projection location; and project the holographic images onto the sample at a rate greater than 1 kHz. Light projection systems for irradiating a sample having light-responsive neurons with holographic images are also described.

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Stanford, CA, United States of America

James Marshel