Lucid, Inc.

International Business Machines Corporation

Lucid Technologies, Inc.

University of Rochester

Caliber Imaging and Diagnostics, Inc.

Other

The General Hospital Corporation

Psc Inc.

Lucii, Inc.

James M Zavislan

Jay M Eastman

Roger J Greenwald

William J Fox

Milind Rajadhyaksha

Kevin P Roesser

Glenn T Sincerbox

Bulent Nihat Kurdi

Timothy Carl Strand

Scott R Grodevant

LeRoy D Dickson

Robert J Hutchison

Robert Dennis Miller

Kurt A Rubin

Hal Jervis Rosen

John Groot

William J Kozlovsky

Francis S Luecke

Milton Russell Latta

Thomas Foster

Anna M Quinn

Aizhong Zhang

Paul Hemmer

Zachary M Eastman

Franklin M Schellenberg

Duncan G Harris

Melbourne E Rabedeau

Richard A Bargerhuff

Andrew Berger

David A Kostizak

Steven H Tomson, Deceased

Kevin Whitcomb

Paul Scott Carney

Arturo Alejandro Canales Benavides

Rahul Asthana

Rebecca Wilson

Frank Luecke

A scanning imaging method includes splitting an optical beam into a reference beam and a scanning beam, generating an interference map, and processing the interference map to produce a reconstructed image of a sample. Generating the interference map includes, for each of a plurality of sections of the sample, generating a respective interference-map element of the interference map by: (i) illuminating the section with the scanning beam to generate a plurality of scattered beams, each scattered beam corresponding to a respective spatial frequency of the scanning beam, (ii) attenuating the reference beam, (iii) generating a plurality of interference signals at least in part by interferometrically combining the plurality of scattered beams and the attenuated reference beam while modulating a phase difference between the reference beam and the plurality of scattered beams, and (iv) detecting the plurality of interference signals to yield the respective interference-map element.

Broadband interferometric confocal microscope

The present disclosure provides systems and methods for characterizing thin films, such as, for example, diagnosis of dry eye syndrome, thin-film optical metrology testing, etc. The present disclosure may be embodied as a tearscope, which is sometimes referred to herein as a Macroscopic Imaging Ellipsometer (MIE) because it may be considered to be an imaging ellipsometer with, for example, a field-of-view on the order of tens of millimeters or more (i.e., macroscopic), compared with conventional imaging ellipsometers, which usually employ microscope objectives and have fields-of-view of up to several hundred microns.

Methods and systems for quantitative ocular surface diagnostics

A cassette for retaining a specimen of surgically exposed tissue from a patient in an orientation that facilitates optical sectioning of the tissue by a confocal microscopic or other optical imaging microscope. The cassette includes a base member having a rigid optically transparent window upon which a tissue specimen is situated, a pliable membrane locatable over a substantial portion of the base member including the window, and an upper member, having an aperture therethrough, which can cover the base member to provide an enclosed cavity between the membrane and the window sealing the tissue specimen therein. The edges of the tissue specimen may be positioned planar against the window and retained in that position by bonds formed between the membrane and window at multiple points or locations around the tissue specimen. The specimen retained in the cavity is imagable by a microscope through the window of the base member.

System for optically sectioning a tissue specimen

A tray or holder for tissue specimens, especially of excised tissue, such as biopsied specimens, is used with a confocal imaging system. The tray may be disposable after imaging of the specimen carried therein or may archive the specimen. A window supports the specimen. Clamps mounted inside the tray restrain the tissue. A compliant bag is mounted outside the tray on one side of a window of the tray on which the specimen is disposed. During imaging the specimen is immersed in a liquid contained in the tray having an index of refraction which closely matches the index of refraction of the tissue. The bag also contains an index matching liquid preferably having the same index as the liquid in the tray. Selecting an immersion liquid, which equals the refractive index of the near surface tissues, minimizes wavefront distortion which may result from an effectively corrugated surface of the specimen.

Tissue specimen holder

An apparatus for determining the location of a radio-opaque marker embedded in an excised specimen includes a radio-opaque marker including an energy beacon that can be embedded in the specimen, an energy beacon controller, at least three detectors, a signal processing component, and a display device. An associated method is disclosed. A method for evaluating a radiographically labeled excised specimen involves determining the location of a radio-opaque marker embedded in the excised specimen, anatomically sectioning the excised specimen, macro-optically segmenting the sectioned specimen into an adipose tissue component, a fibrous tissue component, and an epithelial tissue component, and microscopically examining at least one of the tissue components. An associated apparatus is disclosed.

System for identifying, inspecting, and examining a radiographically labeled specimen

A cassette for retaining a specimen of surgically exposed tissue from a patient in an orientation that facilitates optical sectioning of the tissue by a confocal microscopic or other optical imaging microscope. The cassette includes a base member having a rigid optically transparent window upon which a tissue specimen is situated, a pliable membrane locatable over a substantial portion of the base member including the window, and an upper member, having an aperture therethrough, which can cover the base member to provide an enclosed cavity between the membrane and the window sealing the tissue specimen therein. The edges of the tissue specimen may be positioned planar against the window and retained in that position by bonds formed between the membrane and window at multiple points or locations around the tissue specimen. The specimen retained in the cavity is imagible by a microscope through the window of the base member.

Cassette for facilitating optical sectioning of a retained tissue specimen

An improved system for confocal imaging within dermal tissue of a patient is provided which minimizes instability in confocal images by reducing the relative motion of the tissue with respect to the confocal imaging optics of the system. The system includes a mechanism for maintaining an area of skin tissue under stress by application of force at the edges of the area, and an imaging head coupled to this mechanism for imaging the stressed skin. The mechanism includes a mechanical structure, such as a platen, brace, or attachment, which both supports the imaging head of the system and applies stress to a limited surface area of the tissue to minimize skin motion during confocal imaging.

System and method for confocal imaging within dermal tissue

A cassette for retaining a specimen of surgically exposed tissue from a patient in an orientation that facilitates optical sectioning of the tissue by a confocal microscopic or other optical imaging microscope. The cassette includes a base member having an optically transparent window upon which a tissue specimen is situated, a membrane locatable over a substantial portion of the base member including the window, and an upper member which can cover the base member to provide an enclosed cavity between the membrane and the window sealing the tissue specimen therein. The edges of the tissue specimen may then be positioned planar against the window and retained in that position by bonds formed between the membrane and window around the tissue specimen. The cassette may be part of a confocal imaging system to produce microscopic images of sections of the tissue specimen useful for Mohs surgery.

System for confocal imaging within dermal tissue

Encapsulated tissue is contained in an optically transparent cassette (). The cassette () or an endcap () enclosing the cassette is marked with a fiducial () indicating and corresponding to the location of the excision on the patient's body. An image, which is preferably a representation of a surface of the tissue specimen and the vertical section(s) area of the tissue internal of the specimen and adjacent to a surface thereof, is obtained by an imaging system (). The cassette is moved, preferably in a stage () which rotates the cassette while translating it, so that the head () of the imaging system provides a linear scan in a direction perpendicular to the wall of the cassette (also perpendicular to the surface of the tissue encapsulated in the cassette). The imaging system's display () indicates the morphology at and in proximity to the surface of the specimen as well as the location thereof.

Imaging of surgical biopsies

A system for macroscopic and confocal imaging of tissue having a macroscopic imager for capturing a macroscopic image of the tissue's surface, a confocal imager for capturing one or more optically formed sectional microscopic images on or within tissue, a computer for receiving images from such imagers, and a tissue attachment device in which the macroscopic imager and confocal imager are each individually presented to the tissue utilizing the tissue attachment device in a predefined alignment, such that imaging locations of the confocal imager with respect to the tissue surface spatially correlate with macroscopic image. A user interface is operable on the computer to enable display of the macroscopic image on a display coupled to the computer, and to indicate a region within the macroscopic image associated with the field of view of the tissue imagable by the confocal imager. The user interface enables graphical tracking and targeting of imaging locations of the confocal imager in macroscopic image, and marking on the macroscopic image of the locations of confocal images with respect to the tissue surface.

System for macroscopic and confocal imaging of tissue

A handheld confocal imaging system for in vivo observation of dermal and subdermal tissue allows diagnosis of conditions substantially beneath the surface of the skin. A confocal head has optics which scan the tissue so as to provide images of vertical sections of the tissue. Both two and three dimensional imaging may be provided for diagnosis and location of basal cell carcinomas and melanomas, and so as to enable visualization of tumor borders prior to excision.

Microscopic imaging apparatus and method

A system is provided for marking on a recording medium (), such as a label, the location of imaged tissue with respect to an exposed surface of the tissue (). Tissue is imaged by a microscope () capable of imaging sections of the tissue below the exposed tissue surface through optics. A ring () applied to the surface of the tissue stabilizes the tissue to the optics and localizes a portion of the tissue surface through an aperture in the ring. An actuator () is connected to both the ring and the microscope for moving the ring to adjust the position of the tissue with respect to the optics, thereby allowing an operator of the system to survey different images of tissue sections with the microscope.

System for marking the locations of imaged tissue with respect to the surface of the tissue

A confocal scanning microscope system () using cross polarization effects and an enhancement agent (acetic acid) to enhance confocal microscope reflectance images of the nuclei of BCCs (basal cell carcinomas) and SCCs (squamous cell carcinomas) in the confocal reflectance images of excised tumor slices. The confocal scanning microscope system having a laser () for generating an illumination beam (), a polygon mirror () for scanning the beam to a tissue sample () and for receiving a returned beam from the tissue sample and detector () for detecting the returned beam to form an image. The system further includes a half-wave plate () having a rotatable stage () and a quarter-wave plate () having a rotatable stage () disposed in the optical path of the illumination beam and at least a linear polarizer () having a rotatable stage () disposed in the optical path of the returned beam from the tissue sample.

System and method for enhancing confocal reflectance images of tissue specimens

In order to determine, rapidly and without the delay required by conventional tissue preparation techniques for pathological examination (freezing, sectioning, staining, etc.), whether, an excision, which may be a biopsy sample, is representative of the morphology of interest of whether an excisional biopsy in which the tissue taken completely removes the abnormality is, in either case, the tissue which is desired to be excisioned, the tissue specimen () in encapsulated, preferably as part of the biopsy procedure. The encapsulated tissue is contained in an optically transparent cassette (). The cassette () or an endcap () enclosing the cassette is marked with a fiducial () indicating and corresponding to the location of the excision on the patient's body. An image, which is preferably a representation of a surface of the tissue specimen and the vertical section(s) area of the tissue internal of the specimen and adjacent to a surface thereof, is obtained by means of an electro-optical imaging system (), preferably a confocal laser scanning microscope. The cassette is moved, preferably in a stage () which rotates the cassette while translating it, so that the head () of the confocal microscope (its objective lens) provides a linear scan in a direction perpendicular to the wall of the cassette (also perpendicular to the surface of the tissue encapsulated in the cassette). The display () from the microscope indicates the morphology at and in proximity to the surface of the specimen as well as the location thereof. The pathologist and the surgeon is thereby provided with information concerning the specimen and whether the entire abnormality desired to be removed has indeed been removed and/or whether the biopsy is representative of the body region of interest in the case of a biopsy sample.

Growing community of inventors

Pittsford, NY, United States of America

James M Zavislan