The University of British Columbia

Sonosep Biotech Inc.

Sonosep Technologies Inc.

James M Piret

Anupam Singhal

Carl L G Hansen

Veronique Lecault

Felix Trampler

Douglas G Kilburn

Stefan A Sonderhoff

Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.

System and method for microfluidic cell culture

A method of washing particles in an acoustic chamber includes (a) flowing a suspension of particles in a suspension medium through a standing wave generated in a standing wave volume of the acoustic chamber to accumulate within the acoustic chamber at least some of the particles as raw particle concentrate; and (b) flowing a wash medium through the acoustic chamber to wash within the acoustic chamber at least some of the particles of the raw particle concentrate, and retaining within the acoustic chamber at least some washed particles.

Methods, systems, and apparatuses for acoustically separating and washing particles

Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet.

Particulate material suspended in a fluid is separated and recycled by means of an ultrasonic resonance wave. In a preferred embodiment, the ultrasonic resonance field is generated within a multilayered composite resonator system including a transducer, the suspension and a mirror parallel to each other. Dimensions and frequencies resonant to the whole system but not exciting Eigen-frequencies of transducer and mirror itself are chosen so that thermal dissipation is minimized. Criteria for flow direction and flow rate are defined in order to maintain a high-quality factor of the composite resonator and to achieve a high-separation efficiency. Generally, the process is suitable for all kinds of particles (solid, liquid or gaseous disperse phases) especially for hydrosols (particles in water) and for separation of biological particles such as mammalian, bacterial and plant cells or aggregates. Specialized applications in biotechnology are described including an acoustic filter for mammalian cell bioreactors or the selective retention of viable cells relative to non-viable cells.

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Vancouver, Canada

James M Piret