Dow Agrosciences LLC

Corteva Agriscience LLC

Pioneer Hi-bred International, Inc.

James M Hasler

Megan Sopko

Marc D Zack

James A Strickland

Takashi Yamamoto

Gerald Bryan Watson

Joel Jay Sheets

Gary David Gustafson

Otto Folkerts

Nailah Orr

Ruth Cong

Chaoxian Geng

Jingtong Hou

John P Mathis

James K Petell

Jianquan Li

Deirdre M Kapka-Kitzman

Kitisri Sukhapinda

Vincent L Salgado

Catherine J Clark

Mark Edward Nelson

Scott Chouinard

Naga Kishore Kakani

Song Leng

Kevin R Cook

Claire Marie Bazyk

The disclosure relates to insecticidal toxin resistance management and screening of novel insecticidal toxins. One embodiment relates to the isolation, characterization, compositions, and methods of use relating to polynucleotides encoding novel insecticidal toxin receptors and the polypeptides encoded thereby. The polynucleotides and polypeptides are useful in identifying or designing novel insecticidal toxin receptor ligands including novel insecticidal toxins.

Insecticidal toxin receptors and methods of use

IRDIG35563 vegetative insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed. The invention includes IRDIG35563 variants, fragments and analogs.

Chimeric insecticidal proteins

The invention is drawn to a method for purifying membrane-bound proteins expressed in recombinant insect cells using N-laurosarcosine. The invention is particularly suited for expressing cadherin-type receptors cloned from, European Corn Borer, and expressed in Sf9 insect cells. The method is optionally adapted for use with 6-his tag proteins.

Methods for purifying insect membrane-bound receptor proteins from recombinant production hosts

The present invention is in the field of identification and characterization of novel insecticidal target sites and, in particular, relates to host cells, assays and antibodies thereto.

Assays utilizing nicotinic acetylcholine receptor subunits

Monoclonal antibodies expressed in plant cells bind targeted transit peptides to decrease steady state levels of passenger proteins in plant organelles.

Antibody-mediated down-regulation of plant proteins

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Danville, IN, United States of America

James M Hasler