Gen-probe, Inc.

James M Carrick

Byron J Knight

Gary D Lair

Thanh N Nguyen

Jerzy A Macioszek

James P Light

Christopher Brandon Davis

Florence F Li

Haitao Li

Robert E Heinz

Robert F Scalese

Michael J Gilly

Jeffrey M Linnen

Jeffrey D Chismar

Xianqun Wang

Mark R Kennedy

James P Light, Ii

Sangeetha Vijaysri Nair

Susan K Yamagata

Haito Li

Method and system for quantifying target nucleic acids using real-time amplification and internal calibration adjustment. The invention employs dual reference calibration curves for approximating a complete calibration curve from only a single adjustment calibrator amplified on the instrument that is to be calibrated.

Single-point calibration of a nucleic acid analyzer

Compositions, methods and kits for detecting Chikungunya virus (CHIKV) nucleic acids are for detecting very low levels of the viral nucleic acids using nucleic acid amplification. The kit includes a first primer up to 100 bases long and includes a target-complementary 3' terminal sequence of 15-48 contiguous bases. The first primer optionally may include a first primer 5′ sequence (i.e., an upstream sequence) that is not complementary to CHIKV nucleic acids.

Method for detecting Chikungunya virus

Method of calibrating a nucleic acid analyzer

Nucleic acid oligomers specific for human parvovirus genomic DNA are disclosed. An assay for amplifying and detecting human parvovirus genotypes 1, 2 and 3 nucleic acid in biological specimens is disclosed. Compositions for amplifying and detecting the presence of human parvovirus genotypes 1, 2 and 3 genomic DNA in human biological specimens are disclosed.

Assay for detection of human parvovirus nucleic acid

Compositions, methods and kits for detecting Chikungunya viral nucleic acids. Particularly described are methods for detecting very low levels of the viral nucleic acids using nucleic acid amplification.

Method for detecting chikungunya virus

Dual reference calibration method and system for quantifying polynucleotides

Nucleic acid assays for detecting nucleic acids of Dengue virus serotypes 1-4 derived from 5' NTR.

Dengue virus assay

An automated analyzer for performing multiple diagnostic assays simultaneously includes multiple stations in which discrete aspects of the assay are performed on fluid samples contained in sample vessels. The analyzer includes stations for automatically preparing a sample, incubating the sample, preforming an analyte isolation procedure, ascertaining the presence of a target analyte, and analyzing the amount of a target analyte. An automated receptacle transporting system moves the sample vessels from one station to the next. A method for performing an automated diagnostic assay includes an automated process for isolating and amplifying a target analyte, and, in one embodiment, a method for real-time monitoring of the amplification process.

Method for determining the amount of an analyte in a sample

Method for quantifying analyte polynucleotide in a test sample using real-time amplification and adjustment of a stored calibration curve. The method may be practiced using as few as a single adjustment calibrator to adjust the stored curve. This simplifies the quantitative analysis procedure, while still providing the advantages of internal calibration adjustment to account for variation in amplification reaction efficiency.

Method of quantifying polynucleotides using a stored calibration curve

System for quantifying analyte polynucleotides employs computer-implemented analysis of real-time amplification data using a calibration curve defined by parametric equations.

Parametric system for quantifying analyte polynucleotides

System for analyzing a polynucleotide-containing sample using real-time amplification and monitoring. The system includes a computer or processing device with software for performing a vector analysis of growth curves. The vector analysis advantageously simplifies polynucleotide quantitation by circumventing the need to establish thresholds used for calculating initiation of the growth phase, or to calculate derivatives.

Real-time amplification and monitoring system incorporating vector-based algorithm for analyzing polynucleotide-containing samples

Methods and kits for preparing and adjusting pre-defined master calibration curves of the type used for quantifying analyte polynucleotides by real-time nucleic acid amplification. Particularly disclosed are methods of preparing one or more master calibration curves on one instrument, and then using those master calibration curves on a different instrument.

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San Diego, CA, United States of America

James M Carrick