Life Technologies Corporation

University of California

Other

Hinz, Wolfgang

Rearick, Todd

Bustillo, James

Fife, Keith

Li, Shifeng

James M Bustillo

Jonathan Marc Rothberg

Wolfgang Hinz

Kim L Johnson

Shifeng Li

Jordan Owens

Keith Glen Fife

Mark James Milgrew

Jonathan M Schultz

John Harris Leamon

Todd Rearick

John F Davidson

David Marran

John Matthew Mauro

William Albert Clark

Kristopher Barbee

Antoine Van Oijen

Martin Huber

James Li

Timothy J Brosnihan

Richard M White

Jeremy Gray

Seung Joon Lee

Jonathan O Schultz

Jonathan M Rothberg

Gregory A Campbell

Todd M Rearick

Jonathan C Schultz

Johnathan M Rothberg

Antoine M Van Oijen

In one embodiment, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface, a first opening extending through a first material and through a portion of a second material located on the first material and a second opening extending from the bottom of the first opening to the top of a liner layer located on the upper surface of the floating gate conductor.

Chemical sensor with air via

Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

Methods and apparatus including array of reaction chambers over array of chemFET sensors for measuring analytes

Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

Methods and apparatus for measuring analytes using large scale FET arrays

Methods and apparatus for measuring analytes

Integrated sensor arrays for biological and chemical analysis

In one embodiment, a device is described. The device includes a material defining a reaction region. The device also includes a plurality of chemically-sensitive field effect transistors have a common floating gate in communication with the reaction region. The device also includes a circuit to obtain respective output signals from the chemically-sensitive field effect transistors indicating an analyte within the reaction region.

Chemical sensor array having multiple sensors per well

Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

ChemFET sensor array including overlying array of wells

Chemically-sensitive sensor array device

Growing community of inventors

Castro Valley, CA, United States of America

James M Bustillo