California Institute of Technology

Intrexon Corporation

James Linton

Michael B Elowitz

Manfred Koller

Yaron E Antebi

Long Cai

Gary L Bright

Heidi Klumpe

Mark William Budde

Fredrik Kamme

Amjad Askary

Carlos Lois

Luis Sanchez Guardado

Gustaf Angelborg

Methods and compositions are provided for the selective activation of a BMP-dependent response in certain cell types. Methods include identifying a ligand or ligand combinations as well as cell receptor profiles that result in selectively activating a ligand-dependent response through interactions with ligand receptors on a first cell type that do not activate the ligand-dependent response in a second cell type.

Compositions and methods for programmable sensing and control through combinatorial molecular interactions

Disclosed herein include systems, methods, compositions, and kits for in situ readout of barcodes, such as DNA barcodes. Barcode constructs containing a promoter (e.g., a phage promoter) that is inactive in live cells can be integrated in the genomes of cells. Cells can be fixed, and phage RNA polymerase can be used for transcription of the barcode to RNA transcripts. The RNA transcripts can be detected using, for example, fluorescent imaging and used to determine barcode sequences.

In situ readout of DNA barcodes

Growing community of inventors

Pasadena, CA, United States of America

James Linton