Marshfield Clinic

US Government As Represented by the Secretary of the Army

James L Weber

Mitchel John Doktycz

Russell J Howard

Jeffrey L Chulay

J Steven Hicks

Alan W Thomas

Jeffery A Lyon

Jon P Chudyk

Terry L Rusch

Kim M Fieweger

Jianhong Che

The present invention is directed to a well sampling tape (otherwise known as 'microwell tape' or simply 'tape'), a dispenser for dispensing small volumes of liquid into the wells formed in the tape and a detector for high-throughput sample reading of the liquid dispensed in the individual wells. The present invention is more specifically directed to a bioassay system incorporating the materials listed above.

Apparatus and method for testing and continuously reading low-volume samples

Abundant interspersed repetitive DNA sequences of the form (dC-dA).sub.n.(dG-dT).sub.n have been shown to exhibit length polymorphisms. The polymorphisms can be used to identify individuals as in paternity and forensic testing, and can be used to map genes which are involved in genetic diseases or in other economically important traits. The polynucleotide provided consists of a DNA fragment, preferably .ltoreq.300 base pairs in length containing one or more blocks of tandem dinucleotide repeats (dC-dA).sub.n.(dG-dT).sub.n wherein n.gtoreq.6 and preferably .gtoreq.10.

Length polymorphisms in (dC-dA).sub.n.(dG-dT).sub.n sequences and method

The present invention is directed to a product and process for characterizing the human genome utilizing polymorphic DNA fragments containing (dC-dA).sub.n .multidot.(dG-dT).sub.n. The process comprises identifying polymorphic DNA fragments containing the above tandem repeat sequence followed by characterizing admixtures of genomic material by amplification of fragments containing the tandem repeat sequence using substantially unique and non-polymorphic primer sequences and appropriate separation of the so amplified fragments.

Length polymorphisms in (dC-dA).sub.n .multidot.(dG-dT).sub.n sequences

A method provides peptides that are antigenic determinants identified by antibodies obtained using intact pathogenic agents that present a restricted set of antigens to surveillance by the immune system.

Antigenic determinants recognized by antibodies obtained using a

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Marshfield, WI, United States of America

James L Weber