The United States of America as Represented by the Department of Health

James L Olds

Stephen B Leighton

A two- and three-dimensional autoradiographical imaging system is provided which includes a charge coupled device for detecting the emission of radioactively labeled substances from materials such as tissue samples, brains of humans or animals, or substances used in electrophoresis applications. In a first aspect, a radioactively labeled substance is included in a tissue sample. The tissue sample is sequentially imaged by a charge coupled device and a sectioning tool such as a microtome to produce a plurality of two-dimensional images. A three-dimensional image of the tissue sample is generated by further processing of the plurality of two-dimensional images derived from the charge coupled device. In a further aspect of the invention, a charge coupled device is utilized to provide realtime imaging of metabolic or physiological parameters involved in brain activity. A charge coupled device is positioned adjacent a portion of brain tissue desired to be examined, the brain tissue having a radioactively labeled substance therein for detection by the charge coupled device. A two-dimensional realtime image is produced using the charge coupled device for use in clinical or behavioral studies. In another aspect of the invention, a charge coupled device is utilized in electrophoresis applications to monitor radioactively labeled or tagged substances during electrophoresis. In this application, use of the charge coupled device permits small sample volumes of material to be used during electrophoresis, as well as automatic end point detection.

Method and apparatus for producing an image of a substance using a

A two- and three-dimensional autoradiographical imaging system is provided which includes a charge coupled device for detecting the emission of radioactively labeled substances from materials such as tissue samples, brains of humans or animals, or substances used in electrophoresis applications. In a first aspect, a radioactively labeled substance is included in a tissue sample. The tissue sample is sequentially imaged by a charge coupled device and a sectioning tool such as a microtome to produce a plurality of two-dimensional images. A three-dimensional image of the tissue sample is generated by further processing of the plurality of two-dimensional images derived from the charge coupled device. In a further aspect of the invention, a charge coupled device is utilized to provide realtime imaging of metabolic or physiological parameters involved in brain activity. A charge coupled device is positioned adjacent a portion of brain tissue desired to be examined, the brain tissue having a radioactively labeled substance therein for detection by the charge coupled device. A two-dimensional realtime image is produced using the charge coupled device for use in clinical or behavioral studies.

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Bethesda, MD, United States of America

James L Olds