University of California

James K Nunez

Jennifer A Doudna

Jonathan S Weissman

Luke A Gilbert

Jin Chen

Provided herein are, inter alia, compositions and methods for manipulation of genomes of living organisms.

Compositions and methods for gene editing

The disclosure provides methods and compositions for the integration (insertion) of a donor DNA molecule into a target DNA molecule. In general, the methods include contacting a target DNA molecule with a linear donor DNA molecule and a Cas 1 protein, where the target DNA molecule includes an AT-rich region (e.g., in some cases positioned 5 and within 50 nucleotides of a region that forms a DNA cruciform structure), where the contacting is not in a bacterial or archaeal cell (e.g., the contacting is in vitro outside of a cell, inside of a eukaryotic cell, etc.), and provides for integration of the donor DNA molecule into the target DNA molecule.

Growing community of inventors

Berkeley, CA, United States of America

James K Nunez