E.i. Dupont De Nemours and Company

James John Ragghianti

Quinn Qun Zhu

Howard Glenn Damude

Narendra S Yadav

Zhixiong Xue

Stephen K Picataggio

Hongxiang Zhang

Daniel Joseph Macool

Peter John Gillies

Dana M Walters Pollak

John E Seip

Dana Walters Pollak

Dana M Pollak

Dana Walters M Pollak

Engineered strains of the oleaginous yeastcapable of producing greater than 25% eicosapentaenoic acid (EPA, an &#x3c9;-3 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of EPA. Production host cells are claimed, as are methods for producing EPA within said host cells.

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An engineered strain of the oleaginous yeastcapable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.

Docosahexaenoic acid producing strains of yarrowia lipolytica

Engineered strains of the oleaginous yeastcapable of producing greater than 10% arachidonic acid (ARA, an &#x3c9;-6 polyunsaturated fatty acid) in the total oil fraction are described. These strains comprise various chimeric genes expressing heterologous desaturases, elongases and acyltransferases, and optionally comprise various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of ARA. Production host cells are claimed, as are methods for producing ARA within said host cells.

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Bear, DE, United States of America

James John Ragghianti