Nanosphere, Inc.

Northwestern University

James J Storhoff

Chad A Mirkin

Robert L Letsinger

Robert C Mucic

Robert Elghanian

Thomas Andrew Taton

Viswanadham Garimella

So-Jung Park

Zhi Li

Peter V Schwartz

Susan R Hagenow

Gang Lu

Yijia P Bao

Michael J Senical

Susan Hetzel

Sudhakar S Marla

Susan R Hetzel

Uwe Muller

The invention provides methods of detecting a nucleic acid. The methods comprise contacting the nucleic acid with one or more types of particles having oligonucleotides attached thereto. In one embodiment of the method, the oligonucleotides are attached to nanoparticles and have sequences complementary to portions of the sequence of the nucleic acid. A detectable change (preferably a color change) is brought about as a result of the hybridization of the oligonucleotides on the nanoparticles to the nucleic acid. The invention also provides compositions and kits comprising particles. The invention further provides methods of synthesizing unique nanoparticle-oligonucleotide conjugates, the conjugates produced by the methods, and methods of using the conjugates. In addition, the invention provides nanomaterials and nanostructures comprising nanoparticles and methods of nanofabrication utilizing nanoparticles. Finally, the invention provides a method of separating a selected nucleic acid from other nucleic acids.

Nanoparticles having oligonucleotides attached thereto and uses therefor

The invention provides methods of nanolithography and products therefor and produced thereby. In particular, the invention provides a nanolithographic method referred to as high force nanografting (HFN). HFN utilizes a tip (e.g., a scanning probe microscope (SPM) tip such as an atomic force microscope (AFM) tip) to pattern a substrate passivated with a resist. In the presence of a patterning compound, the tip is used to apply a high force to the substrate to remove molecules of the resist from the substrate, whereupon molecules of the patterning compound are able to attach to the substrate the form the desired pattern.

Nanolithography methods and products therefor and produced thereby

This invention provides polyanionic polymer conjugates containing non-nucleotide polyanionic polymers that are useful in detecting target analytes such as proteins or small molecules. The invention also provides nanoparticles bound to polyanionic polymer conjugates and methods of preparation and use thereof. The polyanionic polymer conjugates have the formula:L-O&#x2014;[PO&#x2014;O-Z-O]&#x2014;PO&#x2014;O&#x2014;Xwherein n ranges from 1 to 200; L represents a moiety comprising a functional group for attaching the polyanion polymer to the nanoparticle surface; Z represents a bridging group, and X represents Q, X' or -Q-X&#x2032;, wherein Q represents a functional group for attaching a recognition probe to the polyanion polymer, and X&#x2032; represents a recognition probe.

Nanoparticle polyanion conjugates and methods of use thereof in detecting analytes

The invention provides nanoparticle-bioconjugate probes that are useful for detecting target analytes such as nucleic acids. The probes of the invention are stable towards heat and resistant to displacement by thiol containing compounds such as DTT (dithiothreitol).

Bioconjugate-nanoparticle probes

Growing community of inventors

Evanston, IL, United States of America

James J Storhoff