Columbia University

Government of the United States of America, As Represented by the Secretary of Commerce

James J Russo

Jingyue Ju

Shiv R Kumar

Sergey M Kalachikov

Minchen Chien

Chuanjuan Tao

Zengmin Li

Xiaoxu Li

Yuan Chang

Patrick S Moore

Isidore S Edelman

Roy A Bohenzky

Shundi Shi

Xin Chen

Min-Kang Hsieh

Steffen Jockusch

Jianyi Ren

Cheng Guo

Irina Morozova

Ece Erturk

Jacob Karl Rosenstein

Ken Shepard

John J Kasianowicz

Carl Williams Fuller

Lin Yu

Joseph W F Robertson

Mirkó Palla

Timothy H Bestor

Youngjin Cho

Anthony Tong

Xuanting Wang

John J Kasianovicz

Joseph WF Robertson

Disclosed herein are highly accurate approaches for single molecule electronic nanopore-based SBS.

High accuracy nanopore-based single molecule sequencing by synthesis with tagged nucleotides

Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing a nucleic acid.

Nucleotide derivatives and methods of use thereof

The invention provides various orthogonal nucleotide analogues and methods for using combinations of said various orthogonal nucleotide analogues for sequencing by synthesis.

Nucleotide analogues and use thereof for nucleic acid sequencing and analysis preliminary class

This disclosure is related to a method of sequencing a single-stranded DNA using deoxynucleotide polyphosphate analogues and translocation of tags from incorporated deoxynucleotide polyphosphate analogues through a nanopore.

DNA sequencing by synthesis using modified nucleotides and nanopore detection

Disclosed herein, inter alia, are nucleotide analogues, and methods of use thereof, having cleavable orthogonal linkers.

3'-O-modified nucleotide analogues with different cleavable linkers for attaching fluorescent labels to the base for DNA sequencing by synthesis

Disclosed herein, inter alia, are compounds, compositions, and methods of use thereof in the sequencing of a nucleic acid.

Synthesis of novel disulfide linker based nucleotides as reversible terminators for DNA sequencing by synthesis

This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

Method of preparation of nanopore and uses thereof

This invention provides nucleotide analogues each of which comprises a tag comprising one or more Forster resonance energy transfer (FRET) acceptor fluorophores, a nucleotide polymerase having one or more FRET donor fluorophores, and methods for sequencing single-stranded.

Flourescene energy transfer-based single molecule/ensemble DNA sequencing by synthesis

This invention provides nucleoside polyphosphate analogues each of which comprises a tag comprising a plurality of Raman-scattering moieties; compounds comprising said nucleoside polyphosphate analogs. This invention also provides nucleotide polymerases with one or more attached and/or conjugated noble metal nanoparticles, wherein the noble metal nanoparticles are surface-enhanced Raman spectroscopy (SERS) substrates thereby creating a region of enhanced sensitivity for surface enhanced Raman spectroscopy (SERS) within or adjacent to the polymerase. This invention also provides a surface with regions of enhanced sensitivity for surface enhanced Raman spectroscopy comprising interspersed rough or nanostructured noble metal surface. This invention also provides methods for determining the sequence of a single stranded DNA or RNA polynucleotide using one or more of nucleoside polyphosphate analogues, polymerase with noble metal nanoparticles, and surface with noble metal.

DNA sequencing by synthesis with nucleotide analogues and Raman detection

This invention provides methods for detecting the presence of a plurality of predetermined compounds in a sample using a plurality of tag moieties and at least one nanopore. This invention also provides methods for determining the quantity of each of a plurality of predetermined compounds in a sample using a plurality of tag moieties and at least one nanopore. This invention further provides methods for detecting interaction of at least two predetermined compounds using at least one tag moiety and at least one nanopore.

Method for detecting multiple predetermined compounds in a sample

Growing community of inventors

New York, NY, United States of America

James J Russo