Bristol-myers Squibb Compnay

Genetic Systems Corporation

James J Blake

Steven G Nadler

Nobuo Monji

Patrick F Coleman

Carol-Ann Cole

Omar K Haffar

Jeffrey S Cleaveland

John P Montana

Wesley J Cosand

Richard C Darveau

Novel polypeptide inhibitors of cytoplasmic protein nuclear translocation are disclosed. The inhibitors are comprised of a signal sequence and at least two nuclear localization sequences. The polypeptides are useful as immunosuppression, antiviral and antitumor agents.

Peptide inhibitors of nuclear protein translocation having nuclear

Peptides which bind to selected ligands are identified by screening peptide libraries which encode a random or controlled collection of amino acid sequences. A rapid and convenient iterative strategy is employed to determine the peptide sequences which bind to the ligand of interest. The peptides so identified can be used as diagnostic or therapeutic agents, or in the design of lead compounds for such uses.

Iterative methods for screening peptide libraries

Peptides immunoreactive with antibodies to native proteins, and which have at least two cysteine residues that contribute to mimicking an epitope of the protein, are prepared with the cysteine thiol groups protected. When deprotected, the peptides have enhanced immunoreactivity. The peptides are particularly useful for detecting antibodies or antigens associated with retroviruses, including the clinically important lymphotropic retroviruses HIV-1, HIV-2, HTLV-I, and HTLV-II.

Cysteine thiol-protected peptides for use in immunoassays

The invention is directed to compositions and methods for treating an infection caused by an organism susceptible to a .beta.-lactam antibiotic comprising two or more active agents. In one embodiment, the composition comprises: (1) a .beta.-lactam antibiotic and (2) a cationic oligopeptide. In another embodiment, the invention is also directed to a composition comprising: (1) a .beta.-lactam antibiotic which inhibits the growth of Enterobacteriaceae bacteria and (2) at least one membrane active substance. Further, the invention is also directed to improved methods for screening potential bactericidal compositions in vitro wherein components of an active complement cascade are included.

Compositions and methods for treating infections caused by organisms

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Seattle, WA, United States of America

James J Blake