Siemens Healthcare Diagnostics Gmbh

James Hnatyszyn

Toumy Guettouche

Thomas Battersby

Marcellinus Beld

Remko Gouw

Carola Van Der Meer

Carola Beatrijs Maria Van Der Meer

The present invention relates to amplification primers with a universal tag and sequencing primers comprising at least one non-natural nucleobase capable of hybridizing to a complementary non-natural nucleobase. The present invention further relates to amplification methods of nucleic acid amplification and sequencing using an amplification primer with a universal tag and sequencing primers, as well as kits and solid supports comprising such primers and tags.

Universal tags with non-natural nucleobases

The present invention is directed to methods and reagents for determining the genotype of a hepatitis C virus (HCV) species present in a test sample. The invention more particularly relates to mixtures of degenerate amplification and sequencing primers, and methods of using such primers, that are complementary to a plurality of HCV species, and are capable of generating nucleotide sequence information for a region of NS5B of HCV that is, for each species, indicative of the type and/or subtype, of the species present in the sample.

Methods and reagents for genotyping HCV

Growing community of inventors

Coral Gables, FL, United States of America

James Hnatyszyn