University of California

University of Illinois

Bp Corporation North America Inc.

Cornell University

Zimitech, Inc.

University of Vienna

Universitat Wien

Charpentier, Emmanuelle

James Harrison Doudna Cate

Jennifer A Doudna

Spencer Charles Knight

Alexandra East-Seletsky

William T Beeson, Iv

Yong-Su Jin

Martin Jinek

Krzysztof Chylinski

Mitchell R O'Connell

N Louise Glass

Huimin Zhao

Chaoguang Tian

Suk-Jin Ha

Soo Rin Kim

Jing Du

Jin Ho Choi

Jonathan M Galazka

Sijin Li

Xiaomin Yang

Michael A Marletta

Emmanuelle Charpentier

Wendell A Lim

Scott C Blanchard

Lei S Qi

Roger Altman

Xin Li

Michael Brian Feldman

Leyi Wang

Kulika Chomvong

Mitchell Ray O′Connell

Elizabeth A Znameroski

Arto Pulk

Michael R Wasserman

Mitchell Ray O'Connell

The present disclosure provides methods for detecting a single-stranded target RNA. The present disclosure provides methods of cleaving a precursor C2c2 guide RNA array into two or more C2c2 guide RNAs. The present disclosure provides a kit for detecting a target RNA in a sample.

Methods and compositions for detecting a target RNA

Disclosed herein are genetically modified microorganisms and related methods for enhanced utilization of oligosaccharides and improved productivity of compounds derived from the metabolism of the oligosaccharides. The microorganisms described herein have altered activities of plasma membrane ATPase protein (PMA1) and/or one or more extracellular glucose sensors, namely, sucrose non-fermenting protein (SNF3), restores glucose transport protein (RGT2), and G protein-coupled receptor 1 protein (GPR1). These genetic modifications provide the microorganisms an increased ability to utilize an oligosaccharide to produce a compound of interest, particularly, tagatose, 2'-fucosyllactose, and psicose. Methods of culturing the microorganisms in the presence of such oligosaccharides to produce the products of interest are also provided.

Engineered microorganisms for enhanced use of oligosaccharides

The present disclosure provides mutant cells for the secretion of proteins and for the degradation of lignocellulosic biomass. Methods for the use of these cells are also provided. Specifically, the utility of combined genetic deletions of &#x3b2;-glucosidases and the catabolite repressor gene creA/cre-1 for protein secretion in fungal and yeast cells is disclosed.

Mutant cells for protein secretion and lignocellulose degradation

The present disclosure relates generally to the production of xylosyl-xylitol oligomers, and more specifically to biological methods for producing xylosyl-xylitol oligomers in host cells.

Methods for production of xylosylxylitol oligomers

The present invention relates to methods to identify molecules that binds in the neomycin binding pocket of a bacterial ribosome using structures of an intact bacterial ribosome that reveal how the ribosome binds tRNA in two functionally distinct states, determined by x-ray crystallography. One state positions tRNA in the peptidyl-tRNA binding site. The second, a fully rotated state, is stabilized by ribosome recycling factor (RRF) and binds tRNA in a highly bent conformation in a hybrid peptidyl/exit (P/E) site. Additionally, the invention relates to various assays, including single-molecule assay for ribosome recycling, and methods to identify compounds that interfere with ribosomal function by detecting newly identified intermediate FRET states using known and novel FRET pairs on the ribosome. The invention also provides vectors and compositions with an N-terminally tagged S13 protein.

Ribosomal targets for antibiotic drug discovery

The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure further relates to methods of increasing transport of cellodextrin into a cell, methods of increasing growth of a cell on a medium containing cellodextrin, methods of co-fermenting cellulose-derived and hemicellulose-derived sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell, methods of increasing transport of a pentose into a cell, methods of increasing growth of a cell on a medium containing pentose sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell.

Methods and compositions for improving sugar transport, mixed sugar fermentation, and production of biofuels

The present disclosure relates to hydrolysis of hexose-&#x3b4;-lactones by use of anextracellular aldonolactonase. In particular the present disclosure relates to compositions including aextracellular aldonolactonase and methods of use thereof.

Extracellular aldonolactonase

Growing community of inventors

Berkeley, CA, United States of America

James Harrison Doudna Cate