The Charles Stark Draper Laboratory, Inc.

James Gillett Truslow

Jeffrey T Borenstein

Nathan Francis Moore

Vishal Tandon

Kenneth T Kotz

Jose A Santos

Alla A Gimbel

Bryan D Teece

Joseph L Charest

Ernest Soonho Kim

Daniel Doty

Louis Kratchman

Abigail June Spencer

Alla Epshteyn

A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.

Apparatus for efficient genetic modification of cells

The present disclosure discusses a system and method that includes a microfluidic device that can be used in either an extracorporeal or implantable configuration. The device supports efficient and safe removal of carbon dioxide from the blood of patients suffering from respiratory disease or injury. The microfluidic device can be a multilayer device that includes gas channels and fluid channels. Distensible membranes within the device can affect a cross-sectional area of the blood channels.

Biomimetic microfluidic device for high efficiency carbon dioxide removal from patients at low blood flow rates

Systems and methods for conducting assays on tissue fragment samples including providing a suspension maintaining pump, and a plurality of fluid reservoirs, wherein the fluid reservoirs are configured to hold a volume of fluid. The fluid reservoirs are fluidically coupled to a microfluidic assay chip, wherein the microfluidic assay chip includes a plurality of parallel assay channels, a first inlet port for introduction of a tissue fragment sample into the microfluidic assay ship, and a second inlet port coupled to the fluid reservoir. Each channel of the microfluidic assay chip also includes a retention barrier configured to trap the tissue fragment sample such that the fluid perfuses through the tissue sample, as well as an outlet port fluidically coupled to a waste receptacle.

Microfluidic system for evaluation of chemotherapeutic and immunotherapeutic drugs

The present disclosure describes a blood oxygenator that includes a checkerboard layout of fluid (e.g., blood) and gas (e.g., oxygen) channels. When viewed as a cross-section through each of the channels of the oxygenator, the checkerboard configuration includes alternating gas and fluid channels in both the x-axis (e.g., in-plane) and in the y-axis (e.g., out-of-plane) directions. The oxygenator described herein reduces manufacturing complexity by using first, second, and third polymer layers that include asymmetrical channel designs. The channel designs include 'open' gas channels, which are exposed to the ambient atmosphere. The oxygenator is placed within a pressure vessel to drive gas into each of the open gas channels, which in some implementations, negates the need for a gas manifold.

Blood oxygenator

Growing community of inventors

Boston, MA, United States of America

James Gillett Truslow