Gen-probe, Inc.

James Garfield Putnam

Daniel L Kacian

Nancy Lau Shen

William M Davis

Michael Garth Riggs

A plasmid for expression of Moloney Murine Leukemia Virus-derived reverse transcriptase in E. coli cells deficient in the expression of indiginous RNAse activity, a method for purification of the recombinant enzyme, and a composition comprising a cloned and purified reverse transcriptase opimized for use in cDNA and nucleic acid amplification procedures.

Highly-purified recombinant reverse transcriptase

Stabilized enzyme compositions for use in nucleic acid amplification. Compositions are provided for the stabilization of one or more enzymes in a single stabilized formulation. Additional compositions incorporate a dried, stabilized enzyme mixture together with necessary cofactors and enzyme substrates in a single container for use upon rehydration. Also disclosed are methods for making and using stabilized enzyme compositions and kits for nucleic acid amplification incorporating the disclosed compositions.

Stabilized enzyme compositions for nucleic acid amplification

Method of making stabilized enzyme compositions for nucleic acid

Stabilized reverse transcriptase and RNA polymerase compositions for use in nucleic acid amplification. Compositions are provided for the stabilization of one or more enzymes in a single stabilized formulation. Additional compositions incorporate a dried, stabilized enzyme mixture together with necessary cofactors and enzyme substrates in a single container for use upon rehydration. Also disclosed are methods for making and using stabilized enzyme compositions and kits for nucleic acid amplification incorporating the disclosed compositions of reverse transcriptase and RNA polymerase lyophilized with a cryoprotectant stabilizing agent including trehalose and polyvinylpyrrolidone.

Growing community of inventors

San Diego, CA, United States of America

James Garfield Putnam