The University of Texas System

University of Rochester

Research, Incorporated

Neurobiotex

James F Leary

Mark A Corio

David G Gorenstein

Bruce A Luxon

Scott R McLaughlin

Xianbin Yang

Christopher J Frederickson

Paul Todd

The present invention includes composition and methods for making and using a combinatorial library to identify modified thioaptamers that bind to, and affect the immune response of a host animal, transcription factors such as IL-6, NF-&#x3ba;B, AP-1 and the like. Composition and methods are also provided for the treatment of viral infections, as well as, vaccines and vaccine adjuvants are provided that modify host immune responses.

Structure based and combinatorially selected oligonucleoside phosphorothioate and phosphorodithioate aptamer targeting AP-1 transcription factors

A system and method for sorting of objects that includes a pathway network that has a plurality of pathways and one or more branch points. A fluid composition including one or more objects can be transported through the pathway network, where one or more of the objects are analyzed and sorted at one or more branch points based on the analysis of the objects.

Flow sorting system and methods regarding same

A data analysis system and/or method, e.g., a data mining/data exploration method, using subtractive clustering is used to explore the similarities and differences between two or more multi-dimensional data sets, e.g., generated using a flow cytometer, an image analysis system, gene expression, or protein microarrays.

Subtractive clustering for use in analysis of data

This invention relates to a method and apparatus for flexibly controlling sorting decisions for a flow cytometer or similar instrument at a purity vs. yield ratio of sorted particles, particularly at high event rates. The system monitors distances between sequential objects to determine if they are in close enough proximity to be within a single resolution unit of the sorting device. The 'friend' or 'foe' status of neighboring events is considered by this system in dealing with detected 'coincident' events. The system operates on information of the desirability to sort the object, i.e. droplet, based upon the properties of the individual object. A storage and retrieval method is provided for making the information available at the time a sort operation based on a sorting logic condition is performed. Accordingly, an easily reconfigurable mode of operation control is provided to allow customized sorting strategies, based on the needs of individual applications or experiments. This system may be included as an integral part of a flow cytometer/cell sorter or similar instrument and may also be provided as an outboard module to such systems.

Method for flexibly sorting particles

A system for sorting particles is arranged such that the presence or absence of multiple parameters is determined for each particle in a flow of particles, and a logic condition is determined based on the multiple parameters. Thereafter, particles are outputted from the system when the logic condition is present. Accordingly, multiparameter high-speed measurements of a rare subpopulation of particles (e.g. biological cells) can be made amidst a larger population of particles with differing characteristics. When attached to a multiparameter flow cytometer/cell sorter and microcomputer, the system allows multiparameter analysis of cells at rates in excess of 100,000 cells/sec. This system can be an outboard module attached to a commercially available or home-built flow cytometer.

System for high-speed measurement and sorting of particles

System for flexibly sorting particles

Method and apparatus for analyzing pulses from optical devices such as flow cytometers to generate indicators of particle dimensional attributes, such as size and shape, as well as orientations in a flow stream of non-spherical particles. Particles are conveyed through a beam of electromagnetic radiation and a photodetector responds to radiation resulting from interaction of the particle with the beam to output a resultant signal in the form of a pulse as a particle enters and passes through the beam. Pulse edge width between two threshold values, preferably established as constant fractions of peak pulse amplitude, is measured as an indication of a particle dimensional attribute.

Growing community of inventors

Rochester, NY, United States of America

James F Leary