Oxford Nanopore Technologies Limited

James Edward Graham

Andrew John Heron

James White

Clive Gavin Brown

Rebecca Victoria Bowen

Daniel George Fordham

Richard Alexander Gutierrez

Melania Slawa Strycharska

The invention provides a method of detecting a target polynucleotide in a sample comprising: (a) contacting the sample with a guide polynucleotide that binds to a sequence in the target polynucleotide and a polynucleotide-guided effector protein, wherein the guide polynucleotide and polynucleotide-guided effector protein form a complex with any target polynucleotide present in the sample; (b) contacting the sample with a membrane comprising a transmembrane pore; (c) applying a potential to the membrane; and (d) monitoring for the presence or absence of an effect resulting from the interaction of the complex with the transmembrane pore to determine the presence or absence of the complex, thereby detecting the target polynucleotide in the sample.

Method for nucleic acid detection by guiding through a nanopore

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Oxford, United Kingdom

James Edward Graham