Dyax Corporation

Wyeth

Other

Thomson Licensing

Genetics Institute, Inc.

James Edward Booth

Brian D Kelley

Jeffrey S Deetz

M Daniel Potter

Jinan Yu

Suresh Vunnum

Paul Brown

Shujun Sun

Ranganathan Godavarti

Mary Beth Switzer

Tianning Yu

Marilous Potter, Legal Representative

Peter S Lee

Gregory David Gudorf

Roger Yeh

Mark Leroy Walker

Samir M Ahmed

Basil Badawiyeh

Lee Douglas Shartzer

Kenneth Rudman

Dan Han Diep

John Frederick Bishop

Timothy Iskra

David Pettigrew

Vasil Nadzakov

Andrew Yoon

Hao Chi Tran

Dana Forte

Genevieve Marie Pinvidic

Jonathan Lee Coffman

Douglas Jason Pickersgill

Jon Coffman

Tim Iskra

The present disclosure is directed towards having a user interface that displays a number of cards or windows. A user can implement a command where the user interface will automatically change the position of the displayed cards to show regions of interest which are the parts of the cards that a user may have interested in. The user interest can be determined in accordance with user preference information. Optionally, cards which do not have regions of interest are removed from a display area and such cards are replaced with new cards that do have regions of interest.

Method for locating regions of interest in a user interface

It is an object of the present invention to provide novel binding molecules for factor VIII and factor VIII-like proteins. Preferred binding molecules of the present invention exhibit not only distinct characteristics for binding of the target factor VIII polypeptides but also specific and desirable characteristics for release (elution) of the target polypeptides. Especially preferred binding molecules according to the invention are short polypeptide sequences, characterized by a stable loop structure.

Binding molecules for human factor VIII and factor VIII-like proteins

This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.

Method of weak partitioning chromatography

Provided herein are methods and compositions for purifying human factor VIII, human factor VIII-like peptide or fragments thereof. The methods comprise immobilizing a binding molecule for human factor VIII, human factor VIII-like protein or fragments thereof to a solid support, contacting the immobilized binding molecule with a solution containing the human factor VIII, human factor VIII-like protein or fragments thereof, and purifying the factor by separating the solution from the solid support.

Methods and compositions for purifying human factor VIII, factor VIII-like proteins or fragments thereof

Methods are provided for purification of Factor VIII polypeptides by affinity chromatography and ion exchange chromatography, in which the eluate from the affinity column is diluted with a solution comprising higher salt concentration, or lower non-polar agent concentration than that of the elution solution, prior to passing the diluted solution through the ion exchange column. The affinity matrix may comprise a monoclonal antibody or a peptide ligand. The methods result in improved purification without significant yield loss.

Methods for producing Factor VIII proteins

Methods for detecting human factor VIII or factor VIII-like polypeptides in and isolating it from solutions such as blood or conditioned media are disclosed, together with reagents suitable for the purpose comprising binding moieties that recognize human factor VIII and/or a factor VIII-like polypeptide and form a binding complex therewith. Preferred polypeptide binding moieties are particularly disclosed.

Polypeptides for binding human factor VIII and fragments of human factor VIII

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Andover, MA, United States of America

James Edward Booth