Middlesex Sciences, Inc.

Epic Therapeutics, Inc.

Other

Transgenic Sciences, Inc.

Corning Glass Works

Sedum Laboratories, Inc.

James E Woiszwillo

Jie Di

Larry Richard Brown

Terrence L Scott

Charles D Blizzard

Judith Sudhalter

Spyros Theodoropulos

Frank J Riske

Natalie S Rudolph

James W Ryan

Fred Rothstein

Roger N Piasio

Compositions for sustained delivery of a biomolecule including an anionic polymer and a cationic polymer which ironically interact with each other and, optionally, with the biomolecule. Methods for making the compositions, including the step of combining the negatively charged polymer with the positively charged polymer to form an ionic complex are also provided. The biomolecule may be complexed with one of the polymers before it is complexed with the oppositely charged polymer. The complex is exposed to conditions that cause the formation of precipitated microcarriers, such as a change in pH or the addition of a complexing molecule. The compositions are preferably formulated into microcarriers.

Ionically formulated biomolecule microcarriers

Methods relating to the field of biochemistry and more specifically relating to microparticles for use in diagnostics, therapeutics, and research are provided.

Macromolecular microparticles and methods of production and use

Microparticles formed by mixing a macromolecule with a polymer at a pH near the isoelectric point of the macromolecule and incubating the mixture in the presence of an energy source for a predetermined length of time. The microparticles are composed of homogeneously distributed, intertwined macromolecule and polymer. Each microparticle allows aqueous fluids to enter and allows solubilized macromolecule and polymer to exit the microparticle and may be formulated to provide a sustained release of macromolecule and polymer from the interior of the microparticle when placed in an appropriate aqueous medium, such as under physiological conditions. Methods of production and methods of use for research, diagnostics and therapeutics are provided.

Method for isolating biomolecules from a biological sample with linear

A macromolecular microparticle composition formed by dehydrating an aqueous macromolecule solution and crosslinking the dehydrated macromolecules with a crosslinking agent while in a liquid phase or with heat. Preferably, the dehydrating agent is a polymer mixture of polyvinylpyrrolidone and polyethylene glycol, the crosslinking reagent is glutaraldehyde, and the macromolecule is a protein, most preferably an immunoglobulin. Methods of use for research, diagnostics and therapeutics are also provided.

Macromolecular microparticles and methods of production

A method and kit for making a polysaccharide-protein Schiff base conjugate. A polysaccharide is oxidized with an oxidizing agent and combined with a protein in the presence of a macromolecular crowding agent to form a Schiff base. Preferably, the macromolecular crowding agent is a soluble linear polymer selected from the group consisting of polyvinylpyrrolidone, polyethylene glycol, dextran, nonylphenol-ethoxylates, polyvinyl alcohol, and mixtures thereof. Most preferably, the macromolecular crowding agent is a mixture of polyvinylpyrrolidone and polyethylene glycol. The microparticles or substantially dissolved microparticles are immunogenic and are useful for inducing an immune response when administered to humans or animals.

Method and kit for making a polysaccharide-protein conjugate

A method and composition for isolating biomolecules from a biological sample wherein the sample is mixed with a soluble, linear polymer, such as polyvinylpyrrolidone, to form a precipitate. The biomolecule of interest is found in the precipitate or is isolated from the supernatant.

A novel chromogenic substrate to peroxidase enzymes is provided which is comprised of a mixture of an adduct formed from a hydrozone and a dienophile, and an aromatic nucleophile. The mixture undergos an oxidative coupling in the presence of peroxidase enzymes and peroxides forming a purple indamine dye. The mixture is also stable and unaffected by oxygen of the air or by hydrogen peroxide.

Chromogenic substrate to peroxidase enzymes

Solvent systems for use in increasing the solubility of 3,3',5,5'-tetramethylbenzidine (TMB) and for making a permanent color record of the results of ELISAs using HRP have been developed. In the improved solvent system containing povidone, 1-ethenyl-2-pyrrolidinone polymers, TMB can be dissolved in a very small quantity of solvent, up to a concentration of 100 to 200 NM, which can then be mixed directly into an aqueous buffer without precipitating the TMB. In the solvent system for making a permanent record, alginic acid (AA), methyl vinyl ether/maleic anhydride copolymer (MVE/MAC), dextran sulfate (DS) and/or carrageenan are added to the aqueous buffer solution, with or without povidone, to form a colored precipitate with the reaction product.

TMB Formulation for soluble and precipitable HRP-ELISA

Solvent systems for use in increasing the solubility of 3,3',5,5'-tetramethylbenzidine (TMB) and for making a permanent color record of the results of ELISAs using HRP have been developed. In the improved solvent system containing povidone, 1-ethenyl-2-pyrrolidinone polymers, TMB can be dissolved in a very small quantity of solvent, up to a concentration of 100 to 200 mM, which can then be mixed directly into an aqueous buffer without precipitating the TMB. In the solvent system for making a permanent record, alginic acid (AA), methyl vinyl ether/maleic anhydride copolymer (MVE/MAC), dextran sulfate (DS) and/or carrageenan are added to the aqueous buffer solution, with or without povidone, to form a colored precipitate with the reaction product.

TMB formulation for soluble and precipitable HRP-ELISA

Method of determining the presence and/or concentration of a polyvalent antigenic substance in a fluid. The method comprises the steps of incubating the fluid with labeled antibodies to the substance to form a first labeled immunochemical complex and then incubating that complex with immobilized antibodies to the substance to form a second labeled complex which is separated from the incubation medium. The amount of label in the second complex provides a means for detecting and/or quantitating the substance in the fluid. By reversing the sequence of incubation steps in a 'two-site' or sandwich assay, greater sensitivity is achieved and an intermediate washing step is eliminated.

Growing community of inventors

Milford, MA, United States of America

James E Woiszwillo