The Penn State Research Foundation

Other

James E Hopper

Gang Grant Peng

Tamara Vyshkina

Vepkhia Pilauri

The invention provides methods for detecting and analyzing protein-protein interactions and agonists and antagonists thereof, detecting and analyzing protein sequences, and regulatable gene expression in multicellular organisms or cells therefrom.

M-GAL: a Gal gene switch-based suite of methods protein analyses and protein expression in multicellular organisms and cells therefrom

This invention provides methods for cytoplasmic detection of protein&#x2014;protein interactions, nuclear export/localization sequences, and galactose-independent inducible Gal4-mediated gene expression through the utilization of GAL regulatory factor, Gal80, and the yeast galactose regulon.

Reagents and methods for detection and characterization of protein-protein interactions, nuclear export and localization sequences and inducible Gal4p-mediated gene expression in yeast

The invention provides methods and recombinant expression constructs for inducing and sustaining high-level production of a recombinant polypeptide in yeast. The invention specifically provides high copy number recombinant expression constructs that express high levels of trans-acting transcription factors that in turn induce expression of a recombinant nucleic acid encoding a heterologous or endogenous recombinant polypeptide. The invention more specifically provides constructs that express galactose-inducible and temperature-sensitive transcription factors. The invention also provides constructs comprising nucleic acids the transcription of which is regulated by the transcription factors expressed by the construct. The invention also provides yeast cells transformed by the recombinant expression constructs of the invention that permit sustained high-level expression of a recombinant polypeptide. The invention further provides methods for producing yeast cells transformed with the recombinant expression constructs of the invention, and methods for inducing high-level expression of a recombinant polypeptide in said transformed yeast cells. The invention also provides such recombinant polypeptides produced by the methods and the transformed yeast cells of the invention.

Growing community of inventors

Hershey, PA, United States of America

James E Hopper