Dsm IP Assets B.v.

Martek Biosciences Corporation

Merial Limited

Sanofi Vaccine Technologies, S.a.s.

James Casey Lippmeier

Kirk Emil Apt

James P Wynn

Paul Warren Behrens

Jon Milton Hansen

Joseph W Pfeifer, Iii

Jaouad Fichtali

John M Thompson

Ross E Zirkle

Jun Wang

James George Metz

David John Simpson

William Robert Barclay

Jerry Kuner

David Christian Martin

Ross Eric Zirkle

David J Kyle

Johannes Andries Roubos

Joyce Pritchard

René Verwaal

F C Thomas Allnutt

Bastien Chevreux

Xuan Guo

Anne-Cecile V Bayne

Anne-Cécile V Bayne

Yelena Betz

Joyce A Pritchard

Jerry M Kuner

Joseph W Iii Pfeifer

Joseph W Pfeifer

Joseph W Pfeifer Iii

The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-Cas system for a Labyrinthulomycetes host cell.

CRISPR-Cas system for an algal host cell

The present invention provides biological oils and methods and uses thereof. The biological oils are preferably produced by heterotrophic fermentation of one or more microorganisms using cellulose-containing feedstock as a main source of carbon. The present invention also provides methods of producing lipid-based biofuels and food, nutritional, and pharmaceutical products using the biological oils.

Biological oils and production and uses thereof

The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention.

Protein production in microorganisms of the phylum labyrinthulomycota

This disclosure concerns a novel modular docosahexaenoic acid (DHA) synthase and recombinant host organisms genetically modified with such synthase and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The disclosure also concerns methods of making and using such organisms as well as products obtained from such organisms.

Production of polyunsaturated fatty acids (PUFAs) using a novel modular docosahexaenoic acid (DHA) synthase

Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.

Production of high levels of DHA-containing biomass in microalgae using modified amounts of chloride and potassium

Polypeptide for improving protein production in microorganisms of the phylum Labyrinthulomycota

Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

Polyunsaturated fatty acid production in heterologous organisms using PUFA polyketide synthase systems

Production of high levels of DHA in microalgae using modified amounts of chloride and potassium

Polynucleotide for improving protein production in microorganisms of the phylum labyrinthulomycota

The present invention is directed to recombinant microalgal cells and their use in production of heterologous hemagglutinin-neuraminidase (HN) polypeptides, as well as compositions and uses thereof.

Production of hemagglutinin-neuraminidase protein in microalgae

Growing community of inventors

Columbia, MD, United States of America

James Casey Lippmeier