University of Toledo

Gene Express, Inc.

Other

Medical College of Ohio

Medical University of Ohio

Medical University of Ohio at Toledo

James C Willey

Erin Crawford

Thomas M Blomquist

David A Weaver

D'Anna N Mullins

Terry Osborn

Robert Zahorchak

Charles Knight

Brad Austermiller

Jiyoun Yeo

Jeffrey P Demuth

Kristy A Warner

Timothy G Graves

Clara M Jackson

Jeffery P DeMuth

Erin L Crawford

D'Aanna N Mullins

Methods for controlling for non-systematic error in an amplification-based next generation sequencing (NGS) library preparation are described, which method includes using an internal amplification control (IAC) sharing identical priming sites to a native nucleic acid target template of interest in a NGS library preparation.

Methods for standardized sequencing of nucleic acids and uses thereof

Methods for standardized sequencing of nucleic acids and uses thereof are described. The identification of genetic information is becoming a key piece of information for the diagnosis and treatment of many diseases. In order to make such diagnostic tool readily available, it is desired that this identification be as efficient and as inexpensive as possible.

Described herein are compositions for evaluating nucleic acids, standardized mixture for assessing amounts of at least one target nucleic acid in a sample, and kits comprising the same.

Standardized mixtures for assessing amounts of target nucleic acids in a sample, and kits therefore

Described herein are materials, methods, and kits enabling accurate and reproducible two-color reverse-transcription real-time quantitative PCR (RT-qPCR) for quality-controlled molecular diagnostic testing of samples that may contain degraded RNA. In certain aspects described herein are materials, methods, and kits for use in the molecular diagnostic testing of lung cancer in FFPE samples and/or fresh-frozen samples. Also described herein are materials and methods to control for inter-experimental variation occurring during two-color RT-qPCR amplification arising from variation in fluorescence specific activity, use of different thermocyclers, and inter-laboratory differences.

Materials and methods for quality-controlled two-color RT-QPCR diagnostic testing of formalin fixed embedded and/or fresh-frozen samples

The present invention is directed to methods and compositions for evaluating nucleic acids, methods of preparing such compositions, and applications and business methods employing such compositions and methods. In particular, the present invention provides business methods for operating a gene expression measurement service.

Compositions of standardized mixtures for determining an amount of a nucleic acid

The present invention relates to methods and compositions for identifying biomarkers that indicate a biological state, in particular cancer or predisposition to cancer.

Cancer risk biomarkers

The present disclosure relates to methods and compositions for identifying biomarkers that indicate a biological state, in particular cancer or predisposition to cancer. Also provided are methods and compositions for identifying a greater risk for a cancer-related condition in an subject.

Cancer risk biomarker

The present invention identifies and quantifies changes in gene expression associated with non-small cell lung cancer NSCLC by examining gene expression in tissue from normal lung and diseased lung. The present invention also identifies and quantifies expression profiles which serve as useful diagnostic markers as well as markers that are useful to monitor disease states, disease progression, drug toxicity, drug efficacy and drug metabolism.

Method for selecting a treatment for non-small cell lung cancer using gene expression profiles

A method measure expression of multiple target genes in a progenitor cell for bronchogenic carcinoma comprising the use of reverse transcription-polymerase chain reaction (RT-PCR) to allow simultaneous expression measurement of the multiple target genes is disclosed.

Method for quantitative measurement of gene expression for identifying individuals at risk for bronchogenic carcinoma

The present invention is directed to methods and compositions for evaluating allelic variations with improved quality controls, methods of preparing such compositions, and applications employing such compositions and methods. In particular, the present invention provides methods and compositions for reducing false positives and/or false negatives in nucleic acid measurements.

Methods and compositions for assessing nucleic acids and alleles

Compositions and methods of use of standardized mixtures for determining an amount of a nucleic acid

The present invention is directed to methods and compositions for assaying one or more analytes in a sample with improved sensitivity, reproducibility and/or lower detection limits, as well as applications employing such compositions and methods. In particular, the present invention provides methods and compositions for assaying one or more non-nucleic acid analytes with one or more other nucleic acid and/or non-nucleic acid analytes that serve as controls.

Methods and compositions for assaying analytes

A method and apparatus for quantitative measurement of gene expression through multiplex competitive reverse transcriptase polymerase chain reaction amplification are disclosed. The method and apparatus are especially useful for analysis of small specimens of cells and tissues.

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Toledo, OH, United States of America

James C Willey