Pioneer Hi-bred International, Inc.

E.i. Dupont De Nemours and Company

Other

Prodigene, Inc.

James C Register, Iii

Christopher Jay Scelonge

Joshua K Young

Cathy Xiaoyan Zhong

M Alejandra Pascual

Albert Laurence Lu

Scott H Diehn

Timothy M Nowatzki

Erin L Crowgey

Douglas S Nubel

John A Howard

Elizabeth E Hood

Derrick Witcher

Terry EuClaire Meyer

Chris L Baszczynski

Matthew Curtis Harmon

Clara Maria Alarcon

David J Peterson

Wesley B Bruce

David A Peterson

A Gururaj Rao

Thomas H Czapla

Sheila E Maddock

The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.

Maize event DP-004114-3 and methods for detection thereof

A method for commercial production of aprotinin entails heterologous expression of the protein in plants, preferably at a level such that aprotinin accounts for at least 0.1% of the total extracted protein. An aprotinin-expressing plant also has potential of increased insecticidal resistance by virtue of producing the protein. A genetic map of the integration locus allows identification of plants derived from the transgenic plant. This approach also reveals genetic loci on a plant chromosome that support high levels of gene expression and can be used as site of integration for expression of other genes of interest.

Commercial production of aprotinin in plants

A method for commercial production of GUS entails heterologous expression of the protein in plants, in native conformation, at an expression level such that avidin represents at least 0.1% of total extracted protein. A genetic map of the integration locus allows for the identification of the GUS-expressing plant. Genetic loci on a plant chromosome are revealed that support high levels of GUS expression and that can be used as a site of integration for high level expression of other genes of interest.

Commercial production of .beta.-glucuronidase in plants

A method for commercial production of avidin entails heterologous expression of the protein in plants, in native conformation, at an expression level such that avidin represents at least 0.1% of total extracted protein. A genetic map of the integration locus allows for the identification of the avidin-expressing plant. Genetic loci on a plant chromosome are revealed that support high levels of avidin expression and that can be used as a site of integration for high level expression of other genes of interest.

Commercial production of avidin in plants

Growing community of inventors

Ames, IA, United States of America

James C Register, Iii