Beckman Instruments, Inc.

Beckman Coulter, Inc.

James C Osborne

Stephen L Pentoney, Jr

Jang B Rampal

Brian D Peterson

Charles A Keenan

Sushma Rampal

A gel pump operated by a stepper motor may be used to deliver fresh gel to one or more capillaries through one or more manifolds and valves. By controlling the valves, fresh gel delivered by the pump will replace the old gel in the capillaries for an automated gel replacement system. In a different setting of the valves, the manifolds may be purged of the old gel prior to delivery of fresh gel to the capillaries. The gel delivery system may also be combined with an electrophoresis system so that sealing connection capable of withstanding high pressure adequate for gel injection need not be frequently broken when the gel is to be replaced. Manifold/reflector assembly is advantageously used in the system that facilitates electrical circuit for electrophoresis and for gel replacement.

Electrophoretic system including means for replacing separation medium

A sample having both positively and negatively charged components may be separated during the same run by injecting a plug of sample at both ends of the capillary and applying an electric field in the capillary, where there is insufficient electroosmotic flow in the capillary to reverse sample migration direction, such as in the case of a gel-filled column.

Capillary electrophoretic system for separation of samples containing

Growing community of inventors

Orange, CA, United States of America

James C Osborne